PMID- 10486255
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 263
IP  - 1
DP  - 1999 Sep 16
TI  - Peflin, a novel member of the five-EF-hand-protein family, is similar to the
      apoptosis-linked gene 2 (ALG-2) protein but possesses nonapeptide repeats in the 
      N-terminal hydrophobic region.
PG  - 68-75
AB  - The calpain small subunits of sorcin, grancalcin, and ALG-2 constitute a family
      of the Ca(2+)-binding proteins with five EF-hand-like motifs (penta-EF-hand
      domain or PEF domain) in their C-terminal regions and hydrophobic domains with
      variable lengths in their N-terminal regions. Searching the human DNA data base
      of expressed sequence tags (EST) revealed novel partial sequences similar to, but
      distinct from, the sequences of the previously known PEF proteins. We isolated a 
      cDNA clone of near full length by 5'- and 3'-RACE (rapid amplification of cDNA
      end) methods and compared the predicted amino acid sequence (284 residues) of the
      novel EF-hand protein, named peflin, with those of known PEF proteins. The PEF
      domain of peflin is most similar to ALG-2 (40.9% identity) among the family,
      particularly in EF-1 (46.2%) and EF-3 (57.1%) regions. Peflin has a longer
      N-terminal hydrophobic domain than any other member of the family, and it
      contains nine nonapeptide (A/PPGGPYGGP) repeats. Western blot analysis
      demonstrated that peflin (30 kDa) was expressed in various nonadherent and
      adherent cultured human cell lines, including Jurkat, HL60, HeLa, and HT1080.
      Peflin may play basic roles in Ca(2+) signaling irrespective of cell types.
CI  - Copyright 1999 Academic Press.
FAU - Kitaura, Y
AU  - Kitaura Y
AD  - Department of Applied Molecular Biosciences, Nagoya University, Furo-cho, Nagoya,
      Chikusa-ku, 464-8601, Japan.
FAU - Watanabe, M
AU  - Watanabe M
FAU - Satoh, H
AU  - Satoh H
FAU - Kawai, T
AU  - Kawai T
FAU - Hitomi, K
AU  - Hitomi K
FAU - Maki, M
AU  - Maki M
LA  - eng
SI  - GENBANK/AB026628
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (PDCD6 protein, human)
RN  - 0 (PEF1 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.22.- (Calpain)
SB  - IM
MH  - Amino Acid Sequence
MH  - Apoptosis
MH  - Apoptosis Regulatory Proteins
MH  - Base Sequence
MH  - Calcium-Binding Proteins/*chemistry/*genetics
MH  - Calpain/*chemistry/*genetics
MH  - Cloning, Molecular
MH  - DNA/genetics
MH  - Escherichia coli/genetics
MH  - Expressed Sequence Tags
MH  - HL-60 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Jurkat Cells
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Recombinant Proteins/chemistry/genetics
MH  - Repetitive Sequences, Amino Acid
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/16 00:00
MHDA- 1999/09/16 00:01
CRDT- 1999/09/16 00:00
PHST- 1999/09/16 00:00 [pubmed]
PHST- 1999/09/16 00:01 [medline]
PHST- 1999/09/16 00:00 [entrez]
AID - 10.1006/bbrc.1999.1189 [doi]
AID - S0006-291X(99)91189-0 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Sep 16;263(1):68-75. doi:
      10.1006/bbrc.1999.1189.