PMID- 10485905
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 19
DP  - 1999 Sep 14
TI  - Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D.
PG  - 10794-9
AB  - Surfactant protein D (SP-D) is an oligomeric C type lectin that promotes
      phagocytosis by binding to microbial surface carbohydrates. A 340-kDa
      glycoprotein (gp-340) has been shown to bind SP-D in the presence of calcium but 
      does so independently of carbohydrate recognition. This protein exists both in a 
      soluble form and in association with the membranes of alveolar macrophages. The
      primary structure of gp-340 has been established by molecular cloning, which
      yielded a 7,686-bp cDNA sequence encoding a polypeptide chain of 2, 413 amino
      acids. The domain organization features 13 scavenger receptor cysteine-rich
      (SRCR) domains, each separated by an SRCR-interspersed domain, except for SRCRs 4
      and 5, which are contiguous. The 13 SRCR domains are followed by two C1r/C1s Uegf
      Bmp1 domains separated by a 14th SRCR domain and a zona pellucida domain. gp-340 
      seems to be an alternative spliced form of DMBT1. Reverse transcription-PCR
      analysis showed that the main sites of synthesis of gp-340 are lung, trachea,
      salivary gland, small intestine, and stomach. Immunohistochemistry revealed
      strong staining for gp-340 in alveolar and other tissue macrophages.
      Immunostaining of the macrophage membrane was either uniform or focal in a way
      that suggested capping, whereas other macrophages showed strong intracellular
      staining within the phagosome/phagolysosome compartments. In some macrophages,
      SP-D and gp-340 were located in the same cellular compartment. Immunoreactive
      gp-340 was also found in epithelial cells of the small intestine and in the ducts
      of salivary glands. The distribution of gp-340 in macrophages is compatible with 
      a role as an opsonin receptor for SP-D.
FAU - Holmskov, U
AU  - Holmskov U
AD  - Department of Immunology and Microbiology, Institute of Medical Biology,
      University of Southern Denmark, Winslowparken 19.1, DK-5000 Odense, Denmark.
      holmskov@imbmed.sku.dk
FAU - Mollenhauer, J
AU  - Mollenhauer J
FAU - Madsen, J
AU  - Madsen J
FAU - Vitved, L
AU  - Vitved L
FAU - Gronlund, J
AU  - Gronlund J
FAU - Tornoe, I
AU  - Tornoe I
FAU - Kliem, A
AU  - Kliem A
FAU - Reid, K B
AU  - Reid KB
FAU - Poustka, A
AU  - Poustka A
FAU - Skjodt, K
AU  - Skjodt K
LA  - eng
SI  - GENBANK/AF159456
SI  - GENBANK/AJ243212
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Glycoproteins)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Proteolipids)
RN  - 0 (Pulmonary Surfactant-Associated Protein D)
RN  - 0 (Pulmonary Surfactant-Associated Proteins)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (glycoprotein 340)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cloning, Molecular
MH  - Glycoproteins/*metabolism
MH  - Humans
MH  - Intestine, Small/metabolism
MH  - Islets of Langerhans/metabolism
MH  - Lung/metabolism
MH  - Macrophages, Alveolar/metabolism
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Mucous Membrane/metabolism
MH  - Opsonin Proteins/*metabolism
MH  - Proteolipids/metabolism
MH  - Pulmonary Surfactant-Associated Protein D
MH  - Pulmonary Surfactant-Associated Proteins
MH  - Pulmonary Surfactants/*metabolism
MH  - Receptors, Immunologic/chemistry/*genetics/physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Salivary Ducts/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
PMC - PMC17962
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
AID - 10.1073/pnas.96.19.10794 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10794-9. doi:
      10.1073/pnas.96.19.10794.