PMID- 10485845
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20190524
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 17
DP  - 1999 Sep 1
TI  - A functional interaction between the histone deacetylase Rpd3 and the corepressor
      groucho in Drosophila development.
PG  - 2218-30
AB  - The Drosophila gene groucho (gro) encodes a transcriptional corepressor that has 
      critical roles in many development processes. In an effort to illuminate the
      mechanism of Gro-mediated repression, we have employed Gro as an affinity reagent
      to purify Gro-binding proteins from embryonic nuclear extracts. One of these
      proteins was found to be the histone deacetylase Rpd3. Protein-protein
      interaction assays suggest that Gro and Rpd3 form a complex in vivo and that they
      interact directly via the glycine/proline rich (GP) domain in Gro. Cell culture
      assays demonstrate that Rpd3 potentiates repression by the GP domain.
      Furthermore, experiments employing a histone deacetylase inhibitor, as well as a 
      catalytically inactive form of Rpd3, imply that histone deacetylase activity is
      required for efficient Gro-mediated repression. Finally, mutations in gro and
      rpd3 have synergistic effects on embryonic lethality and pattern formation. These
      findings support the view that Gro mediates repression, at least in part, by the 
      direct recruitment of the histone deacetylase Rpd3 to the template, where it can 
      modulate local chromatin structure. They also provide evidence for a specific
      role of Rpd3 in early development.
FAU - Chen, G
AU  - Chen G
AD  - Department of Chemistry and Biochemistry, University of California, Los Angeles, 
      California 90095 USA.
FAU - Fernandez, J
AU  - Fernandez J
FAU - Mische, S
AU  - Mische S
FAU - Courey, A J
AU  - Courey AJ
LA  - eng
GR  - R01 GM044522/GM/NIGMS NIH HHS/United States
GR  - GM44522/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (gro protein, Drosophila)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Binding Sites
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Drosophila melanogaster/*embryology/*genetics
MH  - Embryo, Nonmammalian/physiology
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Glycine
MH  - Histone Deacetylases/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Proline
MH  - Protein Biosynthesis
MH  - Repressor Proteins/chemistry/genetics/*metabolism
MH  - Transcription, Genetic
PMC - PMC316998
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
AID - 10.1101/gad.13.17.2218 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Sep 1;13(17):2218-30. doi: 10.1101/gad.13.17.2218.