PMID- 10485494
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 17
DP  - 1999 Sep 1
TI  - Cloning of tumor-associated differentially expressed gene-14, a novel serine
      protease overexpressed by ovarian carcinoma.
PG  - 4435-9
AB  - The family of enzymes known as serine proteases supports many biological
      functions for cancer cells, including activation of growth and angiogenic factors
      and activation of other proteases for invasion and metastasis. In addition, many 
      of these serine proteases are secreted by cells into the extracellular space to
      serve these functions. Therefore, serine proteases are excellent candidate tumor 
      markers. To examine serine proteases expressed by ovarian carcinoma, we designed 
      degenerate PCR primers corresponding to the conserved regions of these genes and 
      used them in reverse transcriptase-PCR experiments with normal and tumor cDNA as 
      a template. The PCR products were subcloned and sequenced, and one of these
      clones was found to encode a novel serine protease, named tumor-associated
      differentially expressed gene-14 (TADG14). Northern blot analysis indicated that 
      the mRNA for TADG14 is 1.4 kb long and that it is highly overexpressed in ovarian
      carcinoma compared with normal ovary. The entire cDNA has been obtained, and
      based on sequence homology, it encodes a 260-amino acid serine protease.
      Semiquantitative PCR indicates that TADG14 is overexpressed in 24 of 40 tumors
      studied. Northern blot data confirm this overexpression, and immunohistochemical 
      staining suggests that this protein is secreted. As such, the TADG14 protease may
      be useful as a diagnostic tool or as a molecular target for therapy.
FAU - Underwood, L J
AU  - Underwood LJ
AD  - Department of Obstetrics, University of Arkansas for Medical Sciences, Little
      Rock 72205-7199, USA.
FAU - Tanimoto, H
AU  - Tanimoto H
FAU - Wang, Y
AU  - Wang Y
FAU - Shigemasa, K
AU  - Shigemasa K
FAU - Parmley, T H
AU  - Parmley TH
FAU - O'Brien, T J
AU  - O'Brien TJ
LA  - eng
SI  - GENBANK/AF055982
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Ovarian Neoplasms/*enzymology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Serine Endopeptidases/analysis/chemistry/*genetics
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Sep 1;59(17):4435-9.