PMID- 10485467
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20041117
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 17
DP  - 1999 Sep 1
TI  - Prostase/KLK-L1 is a new member of the human kallikrein gene family, is expressed
      in prostate and breast tissues, and is hormonally regulated.
PG  - 4252-6
AB  - By using the positional candidate gene approach, we were able to identify a novel
      serine protease gene that maps to chromosome 19q13.3-q13.4. Screening of
      expressed sequence tags allowed us to establish the expression of the gene and
      delineate its genomic organization (GenBank accession no. AF135023). We named
      this gene KLK-L1. Another group, by using a subtraction hybridization method,
      cloned the same gene and named it prostase (GenBank accession nos. AF113140 and
      AF113141). Here, we describe the precise mapping and localization of the
      prostase/KLK-L1 gene between the known genes KLK2 (human glandular kallikrein)
      and zyme (also known as protease M/neurosin). The direction of transcription of
      prostase/KLK-L1 is the same as that of zyme but opposite to that of KLK2 and
      prostate-specific antigen genes. Contrary to the initial impression,
      prostase/KLK-L1 is expressed at high levels not only in prostate tissue but also 
      in testis, mammary gland, adrenals, uterus, thyroid, and salivary glands. We have
      further demonstrated with in vitro experiments with the breast carcinoma cell
      line BT-474 that this gene is expressed and that its expression is up-regulated
      by androgens and progestins. On the basis of information on other genes that are 
      localized in the same region (prostate-specific antigen, KLK2, zyme, and normal
      epithelial cell specific-1 gene), we speculate that prostase/KLK-L1 may be
      involved in the pathogenesis and/or progression of prostate, breast, and possibly
      other malignancies.
FAU - Yousef, G M
AU  - Yousef GM
AD  - Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto,
      Ontario, Canada.
FAU - Obiezu, C V
AU  - Obiezu CV
FAU - Luo, L Y
AU  - Luo LY
FAU - Black, M H
AU  - Black MH
FAU - Diamandis, E P
AU  - Diamandis EP
LA  - eng
SI  - GENBANK/AF113140
SI  - GENBANK/AF113141
SI  - GENBANK/AF135023
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Gonadal Steroid Hormones)
RN  - EC 3.4.21.- (Kallikreins)
SB  - IM
MH  - Base Sequence
MH  - Breast/*enzymology
MH  - Chromosome Mapping
MH  - Female
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Gonadal Steroid Hormones/*pharmacology
MH  - Humans
MH  - Kallikreins/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Prostate/*enzymology
MH  - Sequence Homology
MH  - Tumor Cells, Cultured
EDAT- 1999/09/15 00:00
MHDA- 1999/09/15 00:01
CRDT- 1999/09/15 00:00
PHST- 1999/09/15 00:00 [pubmed]
PHST- 1999/09/15 00:01 [medline]
PHST- 1999/09/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Sep 1;59(17):4252-6.