PMID- 10484328
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20181022
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 3
DP  - 1999 Sep
TI  - Electrically silent potassium channel subunits from human lens epithelium.
PG  - C412-24
LID - 10.1152/ajpcell.1999.277.3.C412 [doi]
AB  - We describe the cloning and characterization of the first human members, hKv9.1
      and hKv9.3, of the electrically silent delayed-rectifying-like K+ channel
      subfamily. Their modulatory effects on the electrically active subfamily member
      hKv2.1 are also quantified. The hKv9 K+ channels were isolated from a human lens 
      epithelium cDNA library, but both hKv9.1 mRNA and hKv9.3 mRNA were found to
      coexist with the mRNA for hKv2.1 in a large number of human tissues. The hKv9.1
      gene is composed of a minimum of five exons, with at least two alternatively
      spliced exons in the 5'-untranslated region (UTR). In contrast, the hKv9.3 gene
      is intronless across the coding region, 3'-UTR, and all of the analyzed 5'-UTR.
      Radiation hybrid mapping localized the hKv9.1 gene to 20q12 and the hKv9.3 gene
      to 2p24. Each electrically silent subunit, when coexpressed with hKv2.l, slows
      deactivation and inactivation compared with hKv2. 1 expressed alone. In addition,
      each results in an increment in the single channel conductance.
FAU - Shepard, A R
AU  - Shepard AR
AD  - Departments of Physiology/Biophysics and Ophthalmology, Mayo Foundation,
      Rochester, Minnesota 55905, USA.
FAU - Rae, J L
AU  - Rae JL
LA  - eng
SI  - GENBANK/AF043472
SI  - GENBANK/AF043473
GR  - EY-03282/EY/NEI NIH HHS/United States
GR  - EY-06005/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Delayed Rectifier Potassium Channels)
RN  - 0 (KCNS1 protein, human)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Voltage-Gated)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Cricetinae
MH  - Delayed Rectifier Potassium Channels
MH  - Electrophysiology
MH  - Epithelium/metabolism
MH  - Humans
MH  - Lens, Crystalline/*metabolism
MH  - Molecular Sequence Data
MH  - Potassium Channels/genetics/metabolism/*physiology
MH  - *Potassium Channels, Voltage-Gated
MH  - Tissue Distribution
MH  - Transfection
EDAT- 1999/09/14 00:00
MHDA- 1999/09/14 00:01
CRDT- 1999/09/14 00:00
PHST- 1999/09/14 00:00 [pubmed]
PHST- 1999/09/14 00:01 [medline]
PHST- 1999/09/14 00:00 [entrez]
AID - 10.1152/ajpcell.1999.277.3.C412 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Sep;277(3):C412-24. doi: 10.1152/ajpcell.1999.277.3.C412.