PMID- 10483362
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20061115
IS  - 0031-8655 (Print)
IS  - 0031-8655 (Linking)
VI  - 70
IP  - 3
DP  - 1999 Sep
TI  - UVB and gamma-radiation induce the expression of mRNAs encoding the ribosomal
      subunit L13A in rat keratinocytes.
PG  - 341-7
AB  - Ultraviolet B radiation produces an array of cellular perturbations in the skin. 
      We isolated a keratinocyte cDNA encoding the rat 60S ribosomal subunit protein
      L13a following differential cDNA library screening with UVB-enriched probes. In
      contrast to the reported structure of liver L13a, the keratinocyte L13a cDNA
      contains a longer 3'-untranslated region. Northern blot analysis detected two
      L13a mRNA transcripts, approximately 800 bp and approximately 1.2 kb, in
      keratinocytes and a variety of rat tissues. Both L13a mRNA transcripts were
      induced by UVB irradiation, forskolin and gamma-irradiation. In contrast, no
      induction of L13a mRNA transcript levels was observed following exposure of
      keratinocytes to 12-O-tetradecanoylphorbol-13-acetate, serum and the DNA
      damage-inducing agents methyl methanesulfonate or 4-nitroquinoline-N-oxide. These
      observations suggest that increased expression of ribosomal subunit genes may be 
      a molecular component of the keratinocyte response to UVB in particular and not
      part of a nonspecific response to DNA damage.
FAU - Shahmolky, N
AU  - Shahmolky N
AD  - Department of Clinical Biochemistry, Women's College Hospital, University of
      Toronto, Canada.
FAU - Lefebvre, D L
AU  - Lefebvre DL
FAU - Poon, R
AU  - Poon R
FAU - Bai, Y
AU  - Bai Y
FAU - Sharma, M
AU  - Sharma M
FAU - Rosen, C F
AU  - Rosen CF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Photochem Photobiol
JT  - Photochemistry and photobiology
JID - 0376425
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (RPL38 protein, rat)
RN  - 0 (Ribosomal Proteins)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Gamma Rays
MH  - Gene Expression Regulation/*radiation effects
MH  - Keratinocytes/*metabolism/*radiation effects
MH  - RNA, Messenger/*biosynthesis/isolation & purification
MH  - Rats
MH  - Ribosomal Proteins/*biosynthesis/genetics
MH  - *Ultraviolet Rays
EDAT- 1999/09/14 00:00
MHDA- 1999/09/14 00:01
CRDT- 1999/09/14 00:00
PHST- 1999/09/14 00:00 [pubmed]
PHST- 1999/09/14 00:01 [medline]
PHST- 1999/09/14 00:00 [entrez]
PST - ppublish
SO  - Photochem Photobiol. 1999 Sep;70(3):341-7.