PMID- 10481931
OWN - NLM
STAT- MEDLINE
DCOM- 19991006
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 285
IP  - 1-2
DP  - 1999 Jul
TI  - Cloning and sequence of partial cDNAs encoding the human type V and VI adenylyl
      cyclases and subsequent RNA-quantification in various tissues.
PG  - 155-61
AB  - Adenylyl cyclases (Acs) and VI are the predominant form of Acs in mammalian heart
      where they are part of the beta-adrenergic pathway. Up to now, the sequences for 
      both enzymes from human tissues have not yet been reported. We investigated the
      mRNA expression AC V and VI in human colon, heart, liver, lung and MNL. Partial
      cDNAs of human types V and VI Acs were amplified by PCR using oligonucleotides
      derived from the cytoplasmatic domain sequences of the corresponding enzymes from
      dog heart. Primers derived from the human sequence were used to detect the mRNAs 
      corresponding to both Acs. For quantification of mRNAs we constructed internal
      standards for competitive quantitative reverse transcriptase PCR (RT-PCR). Both
      types of transcripts could be found in all investigated tissues except MNL where 
      only type VI could be detected. Further we demonstrated a more than 60 times
      higher amount of AC V-mRNA in human heart compared to AC VI-mRNA.
FAU - Raimundo, S
AU  - Raimundo S
AD  - Department of Clinical Pathology, Robert-Bosch-Krankenhaus and Dr. Margarete
      Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany.
FAU - Giray, J
AU  - Giray J
FAU - Volff, J N
AU  - Volff JN
FAU - Schwab, M
AU  - Schwab M
FAU - Altenbuchner, J
AU  - Altenbuchner J
FAU - Ratge, D
AU  - Ratge D
FAU - Wisser, H
AU  - Wisser H
LA  - eng
SI  - GENBANK/U65473
SI  - GENBANK/U65474
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Dogs
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1999/09/11 00:00
MHDA- 1999/09/11 00:01
CRDT- 1999/09/11 00:00
PHST- 1999/09/11 00:00 [pubmed]
PHST- 1999/09/11 00:01 [medline]
PHST- 1999/09/11 00:00 [entrez]
AID - S0009-8981(99)00067-4 [pii]
AID - 10.1016/s0009-8981(99)00067-4 [doi]
PST - ppublish
SO  - Clin Chim Acta. 1999 Jul;285(1-2):155-61. doi: 10.1016/s0009-8981(99)00067-4.