PMID- 10481053
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 458
IP  - 2
DP  - 1999 Sep 17
TI  - Characterization of testicular mouse glucosamine 6-phosphate deaminase (GNPDA).
PG  - 141-4
AB  - Mammalian glucosamine 6-phosphate deaminase (GNPDA) was first detected in hamster
      spermatozoa. To further elucidate its role, we have cloned mouse GNPDA and
      produced a polyclonal rabbit anti-GNPDA antibody. This antibody recognized a 33
      kDa protein in soluble extracts from mouse brain, liver, kidney, muscle, ovary,
      testis and sperm. Immunofluorescent analysis of the localization of GNPDA in male
      reproductive tissue revealed its presence in spermatids and in spermatozoa. In
      spermatids, GNPDA localized close to the developing acrosome vesicle and in
      spermatozoa close to the acrosomal region. Following the induction of the
      acrosome reaction, GNPDA fluorescence in spermatozoa was either reduced or GNPDA 
      was absent. These data suggest that GNPDA might play a role in the acrosome
      reaction.
FAU - Montag, M
AU  - Montag M
AD  - Department of Endocrinology and Reproductive Medicine, University of Bonn,
      Germany. m.montag@uni-bonn.de
FAU - van der Ven, K
AU  - van der Ven K
FAU - Dorbecker, C
AU  - Dorbecker C
FAU - van der Ven, H
AU  - van der Ven H
LA  - eng
SI  - GENBANK/AF088903
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Immune Sera)
RN  - EC 3.5.99.6 (glucosamine-6-phosphate isomerase)
RN  - EC 5.3.1.- (Aldose-Ketose Isomerases)
SB  - IM
MH  - Acrosome/enzymology/physiology
MH  - Aldose-Ketose Isomerases/*chemistry/genetics/isolation & purification/physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Fluorescent Antibody Technique, Indirect
MH  - Immune Sera/metabolism
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics
MH  - Sequence Analysis
MH  - Sperm Capacitation
MH  - Spermatozoa/enzymology/physiology
MH  - Testis/*enzymology
EDAT- 1999/09/11 00:00
MHDA- 1999/09/11 00:01
CRDT- 1999/09/11 00:00
PHST- 1999/09/11 00:00 [pubmed]
PHST- 1999/09/11 00:01 [medline]
PHST- 1999/09/11 00:00 [entrez]
AID - S0014-5793(99)01141-2 [pii]
AID - 10.1016/s0014-5793(99)01141-2 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Sep 17;458(2):141-4. doi: 10.1016/s0014-5793(99)01141-2.