PMID- 10480937
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 38
DP  - 1999 Sep 17
TI  - Characterization of the targeting, binding, and phosphorylation site domains of
      an A kinase anchor protein and a myristoylated alanine-rich C kinase
      substrate-like analog that are encoded by a single gene.
PG  - 27201-10
AB  - A novel Drosophila A kinase anchor protein, Drosophila A kinase anchor protein
      200 (DAKAP200), is predicted to be involved in routing, mediating, and
      integrating signals carried by cAMP, Ca(2+), and diacylglycerol (Li, Z., Rossi,
      E. A., Hoheisel, J. D., Kalderon, D., and Rubin, C. S. (1999) J. Biol. Chem. 274,
      27191-27200). Experiments designed to assess this hypothesis now (a) establish
      the function, boundaries and identity of critical amino acids of the protein
      kinase AII (PKAII) tethering site of DAKAP200; (b) demonstrate that residues
      119-148 mediate binding with Ca(2+)-calmodulin and F-actin; (c) show that a
      polybasic region of DAKAP200 is a substrate for protein kinase C; (d) reveal that
      phosphorylation of the polybasic domain regulates affinity for F-actin and
      Ca(2+)-calmodulin; and (e) indicate that DAKAP200 is myristoylated and that this 
      modification promotes targeting of DAKAP200 to plasma membrane. DeltaDAKAP200, a 
      second product of the DAKAP200 gene, cannot tether PKAII. However, DeltaDAKAP200 
      is myristoylated and contains a phosphorylation site domain that binds
      Ca(2+)-calmodulin and F-actin. An atypical amino acid composition, a high level
      of negative charge, exceptional thermostability, unusual hydrodynamic properties,
      properties of the phosphorylation site domain, and a calculated M(r) of 38,000
      suggest that DeltaDAKAP200 is a new member of the myristoylated alanine-rich C
      kinase substrate protein family. DAKAP200 is a potentially mobile, chimeric A
      kinase anchor protein-myristoylated alanine-rich C kinase substrate protein that 
      may facilitate localized reception and targeted transmission of signals carried
      by cAMP, Ca(2+), and diacylglycerol.
FAU - Rossi, E A
AU  - Rossi EA
AD  - Department of Molecular Pharmacology, Atran Laboratories, Albert Einstein College
      of Medicine, Bronx, New York 10461, USA.
FAU - Li, Z
AU  - Li Z
FAU - Feng, H
AU  - Feng H
FAU - Rubin, C S
AU  - Rubin CS
LA  - eng
GR  - DK07513/DK/NIDDK NIH HHS/United States
GR  - GM07260/GM/NIGMS NIH HHS/United States
GR  - GM22792/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (A Kinase Anchor Proteins)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Akap200 protein, Drosophila)
RN  - 0 (Carrier Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 125267-21-2 (Myristoylated Alanine-Rich C Kinase Substrate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - A Kinase Anchor Proteins
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium/metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Chickens
MH  - Cyclic AMP/metabolism
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Myristoylated Alanine-Rich C Kinase Substrate
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - Protein Kinase C/genetics/*metabolism
MH  - Proteins/genetics/*metabolism
MH  - Signal Transduction
MH  - Structure-Activity Relationship
EDAT- 1999/09/10 00:00
MHDA- 1999/09/10 00:01
CRDT- 1999/09/10 00:00
PHST- 1999/09/10 00:00 [pubmed]
PHST- 1999/09/10 00:01 [medline]
PHST- 1999/09/10 00:00 [entrez]
AID - 10.1074/jbc.274.38.27201 [doi]
AID - S0021-9258(19)55144-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 17;274(38):27201-10. doi: 10.1074/jbc.274.38.27201.