PMID- 10480910
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 38
DP  - 1999 Sep 17
TI  - Natural splicing of exon 2 of human interleukin-15 receptor alpha-chain mRNA
      results in a shortened form with a distinct pattern of expression.
PG  - 26978-84
AB  - We report the existence of eight different interleukin-15 receptor alpha-chain
      (IL-15Ralpha) transcripts resulting from exon-splicing mechanisms within the
      IL-15Ralpha gene. Two main classes of transcripts can be distinguished that do or
      do not (Delta2 isoforms) contain the exon 2-coding sequence. Both classes were
      expressed in numerous cell lines and tissues (including peripheral blood
      lymphocytes) at comparable levels and could be transcribed in COS-7 cells, and
      the proteins were expressed at the cell surface. Both receptor forms displayed
      numerous glycosylation states, reflecting differential usage of a single
      N-glycosylation site as well as extensive O-glycosylations. Whereas IL-15Ralpha
      bound IL-15 with high affinity, Delta2IL-15Ralpha was unable to bind IL-15, thus 
      revealing the indispensable role of the exon 2-encoded domain in cytokine
      binding. A large proportion of IL-15Ralpha was expressed at the nuclear membrane 
      with some intranuclear localization, supporting a potential direct action of the 
      IL-15.IL-15Ralpha complex at the nuclear level. In sharp contrast,
      Delta2IL-15Ralpha was found only in the non-nuclear membrane compartments,
      indicating that the exon 2-encoded domain (which is shown to contain a potential 
      nuclear localization signal) plays an important role in receptor
      post-translational routing. Together, our data indicate that exon 2 splicing of
      human IL-15Ralpha is a natural process that might play regulatory roles at
      different levels.
FAU - Dubois, S
AU  - Dubois S
AD  - Groupe de Recherche Cytokines et Recepteurs, Unite INSERM 463, Institut de
      Biologie, 9 Quai Moncousu, 44035 Nantes Cedex 01, France.
FAU - Magrangeas, F
AU  - Magrangeas F
FAU - Lehours, P
AU  - Lehours P
FAU - Raher, S
AU  - Raher S
FAU - Bernard, J
AU  - Bernard J
FAU - Boisteau, O
AU  - Boisteau O
FAU - Leroy, S
AU  - Leroy S
FAU - Minvielle, S
AU  - Minvielle S
FAU - Godard, A
AU  - Godard A
FAU - Jacques, Y
AU  - Jacques Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (IL15RA protein, human)
RN  - 0 (Interleukin-15)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-15)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell Line
MH  - *Exons
MH  - *Gene Expression Regulation
MH  - Glycosylation
MH  - Humans
MH  - Interleukin-15/metabolism
MH  - Polymerase Chain Reaction
MH  - *RNA Splicing
MH  - RNA, Messenger/metabolism
MH  - Receptors, Interleukin-15
MH  - Receptors, Interleukin-2/*genetics
MH  - U937 Cells
EDAT- 1999/09/10 00:00
MHDA- 1999/09/10 00:01
CRDT- 1999/09/10 00:00
PHST- 1999/09/10 00:00 [pubmed]
PHST- 1999/09/10 00:01 [medline]
PHST- 1999/09/10 00:00 [entrez]
AID - 10.1074/jbc.274.38.26978 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 17;274(38):26978-84. doi: 10.1074/jbc.274.38.26978.