PMID- 10480909
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 38
DP  - 1999 Sep 17
TI  - Glypican-6, a new member of the glypican family of cell surface heparan sulfate
      proteoglycans.
PG  - 26968-77
AB  - The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan
      sulfate proteoglycans. Mutations in dally, a gene encoding a Drosophila glypican,
      and in GPC3, the gene for human glypican-3, implicate glypicans in the control of
      cell growth and division. So far, five members of the glypican family have been
      identified in vertebrates. By sequencing expressed sequence tag clones and
      products of rapid amplifications of cDNA ends, we identified a sixth member of
      the glypican family. The glypican-6 mRNA encodes a protein of 555 amino acids
      that is most homologous to glypican-4 (identity of 63%). Expression of this
      protein in Namalwa cells shows a core protein of approximately 60 kDa that is
      substituted with heparan sulfate only. GPC6, the gene encoding human glypican-6, 
      contains nine exons. Like GPC5, the gene encoding glypican-5, GPC6 maps to
      chromosome 13q32. Clustering of the GPC5/GPC6 genes on chromosome 13q32 is
      strongly reminiscent of the clustering of the GPC3/GPC4 genes on chromosome Xq26 
      and suggests GPCs arose from a series of gene and genome duplications. Based on
      similarities in sequence and gene organization, glypican-1, glypican-2,
      glypican-4, and glypican-6 appear to define a subfamily of glypicans, differing
      from the subfamily comprising so far glypican-3 and glypican-5. Northern
      blottings indicate that glypican-6 mRNA is widespread, with prominent expressions
      in human fetal kidney and adult ovary. In situ hybridization studies localize
      glypican-6 to mesenchymal tissues in the developing mouse embryo. High
      expressions occur in smooth muscle cells lining the aorta and other major blood
      vessels and in mesenchymal cells of the intestine, kidney, lung, tooth, and
      gonad. Growth factor signaling in these tissues might in part be regulated by the
      presence of glypican-6 on the cell surface.
FAU - Veugelers, M
AU  - Veugelers M
AD  - Laboratory for Glycobiology, Center for Human Genetics, University of Leuven,
      B-3000, Belgium.
FAU - De Cat, B
AU  - De Cat B
FAU - Ceulemans, H
AU  - Ceulemans H
FAU - Bruystens, A M
AU  - Bruystens AM
FAU - Coomans, C
AU  - Coomans C
FAU - Durr, J
AU  - Durr J
FAU - Vermeesch, J
AU  - Vermeesch J
FAU - Marynen, P
AU  - Marynen P
FAU - David, G
AU  - David G
LA  - eng
SI  - GENBANK/AF105267
SI  - GENBANK/AF105268
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (GPC6 protein, human)
RN  - 0 (Glypicans)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Glypicans
MH  - Heparan Sulfate Proteoglycans/*genetics
MH  - Heparitin Sulfate/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Alignment
EDAT- 1999/09/10 00:00
MHDA- 1999/09/10 00:01
CRDT- 1999/09/10 00:00
PHST- 1999/09/10 00:00 [pubmed]
PHST- 1999/09/10 00:01 [medline]
PHST- 1999/09/10 00:00 [entrez]
AID - 10.1074/jbc.274.38.26968 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 17;274(38):26968-77. doi: 10.1074/jbc.274.38.26968.