PMID- 10480894
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 38
DP  - 1999 Sep 17
TI  - RGS1 is expressed in monocytes and acts as a GTPase-activating protein for
      G-protein-coupled chemoattractant receptors.
PG  - 26860-8
AB  - The leukocyte response to chemoattractants is transduced by the interaction of
      transmembrane receptors with GTP-binding regulatory proteins (G-proteins). RGS1
      is a member of a protein family constituting a newly appreciated and large group 
      of proteins that act as deactivators of G-protein signaling pathways by
      accelerating the GTPase activity of G-protein alpha subunits. We demonstrate here
      that RGS1 is expressed in human monocytes; by immunofluorescence and subcellular 
      fractionation RGS1 was localized to the plasma membrane. By using a mixture of
      RGS1 and plasma membranes, we were able to demonstrate GAP activity of RGS1 on
      receptor-activated G-proteins; RGS1 did not affect ligand-stimulated GDP-GTP
      exchange. We found that RGS1 desensitizes a variety of chemotactic receptors
      including receptors for N-formyl-methionyl-leucyl-phenylalanine, leukotriene B4, 
      and C5a. Interaction of RGS proteins and ligand-induced G-protein signaling can
      be demonstrated by determining GTPase activity using purified RGS proteins and
      plasma membranes.
FAU - Denecke, B
AU  - Denecke B
AD  - Institut fur Medizinische Mikrobiologie, Medizinische Hochschule Hannover,
      Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany.
FAU - Meyerdierks, A
AU  - Meyerdierks A
FAU - Bottger, E C
AU  - Bottger EC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RGS Proteins)
RN  - 0 (RGS1 protein, human)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Peptide)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rab3 GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - CHO Cells
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - GTP Phosphohydrolases/*metabolism
MH  - GTP-Binding Proteins/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Humans
MH  - Microscopy, Confocal
MH  - Monocytes/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - *Protein Biosynthesis
MH  - *RGS Proteins
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, Peptide/*metabolism
MH  - Transfection
MH  - U937 Cells
MH  - rab3 GTP-Binding Proteins
EDAT- 1999/09/10 00:00
MHDA- 1999/09/10 00:01
CRDT- 1999/09/10 00:00
PHST- 1999/09/10 00:00 [pubmed]
PHST- 1999/09/10 00:01 [medline]
PHST- 1999/09/10 00:00 [entrez]
AID - 10.1074/jbc.274.38.26860 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 17;274(38):26860-8. doi: 10.1074/jbc.274.38.26860.