PMID- 10477764
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20211203
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 146
IP  - 5
DP  - 1999 Sep 6
TI  - Afadin: A key molecule essential for structural organization of cell-cell
      junctions of polarized epithelia during embryogenesis.
PG  - 1117-32
AB  - Afadin is an actin filament-binding protein that binds to nectin, an
      immunoglobulin-like cell adhesion molecule, and is colocalized with nectin at
      cadherin-based cell-cell adherens junctions (AJs). To explore the function of
      afadin in cell-cell adhesion during embryogenesis, we generated afadin(-/-) mice 
      and embryonic stem cells. In wild-type mice at embryonic days 6.5-8.5, afadin was
      highly expressed in the embryonic ectoderm and the mesoderm, but hardly detected 
      in the extraembryonic regions such as the visceral endoderm. Afadin(-/-) mice
      showed developmental defects at stages during and after gastrulation, including
      disorganization of the ectoderm, impaired migration of the mesoderm, and loss of 
      somites and other structures derived from both the ectoderm and the mesoderm.
      Cystic embryoid bodies derived from afadin(-/-) embryonic stem cells showed
      normal organization of the endoderm but disorganization of the ectoderm.
      Cell-cell AJs and tight junctions were improperly organized in the ectoderm of
      afadin(-/-) mice and embryoid bodies. These results indicate that afadin is
      highly expressed in the ectoderm- derived cells during embryogenesis and plays a 
      key role in proper organization of AJs and tight junctions of the highly
      expressing cells, which is essential for proper tissue morphogenesis.
FAU - Ikeda, W
AU  - Ikeda W
AD  - Department of Molecular Biology and Biochemistry, Osaka University Graduate
      School of Medicine/Faculty of Medicine, Osaka 565-0871, Japan.
FAU - Nakanishi, H
AU  - Nakanishi H
FAU - Miyoshi, J
AU  - Miyoshi J
FAU - Mandai, K
AU  - Mandai K
FAU - Ishizaki, H
AU  - Ishizaki H
FAU - Tanaka, M
AU  - Tanaka M
FAU - Togawa, A
AU  - Togawa A
FAU - Takahashi, K
AU  - Takahashi K
FAU - Nishioka, H
AU  - Nishioka H
FAU - Yoshida, H
AU  - Yoshida H
FAU - Mizoguchi, A
AU  - Mizoguchi A
FAU - Nishikawa, S
AU  - Nishikawa S
FAU - Takai, Y
AU  - Takai Y
LA  - eng
SI  - GENBANK/AF172447
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actins)
RN  - 0 (Afdn protein, mouse)
RN  - 0 (Cadherins)
RN  - 0 (Cdh2 protein, mouse)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Microfilament Proteins)
RN  - 0 (afadin)
RN  - EC 3.6.4.1 (Myosins)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Brain/metabolism
MH  - Cadherins
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/metabolism
MH  - *Cell Polarity
MH  - Embryo, Mammalian/*cytology/metabolism
MH  - Epithelial Cells/*cytology/metabolism
MH  - Female
MH  - Gastrula/cytology/metabolism
MH  - Gene Deletion
MH  - Genotype
MH  - Germ Layers/cytology/metabolism
MH  - Kinesins
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Microfilament Proteins/genetics/*metabolism
MH  - Morphogenesis
MH  - Myosins
MH  - Stem Cells/cytology/metabolism
MH  - Tight Junctions/*metabolism
PMC - PMC2169488
EDAT- 1999/09/09 00:00
MHDA- 1999/09/09 00:01
CRDT- 1999/09/09 00:00
PHST- 1999/09/09 00:00 [pubmed]
PHST- 1999/09/09 00:01 [medline]
PHST- 1999/09/09 00:00 [entrez]
AID - 10.1083/jcb.146.5.1117 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Sep 6;146(5):1117-32. doi: 10.1083/jcb.146.5.1117.