PMID- 10477728
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20080716
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 94
IP  - 6
DP  - 1999 Sep 15
TI  - Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible
      transcriptional repressor, is involved in osteoclastogenesis.
PG  - 1987-97
AB  - The differentiation of osteoclasts is regulated by transcription factors
      expressed in cells of osteoclast lineage. We isolated here a potential
      transcription factor from a cDNA library of an enriched population of
      preosteoclasts and osteoclasts. The cDNA encodes a protein with N-terminal POZ
      domain and C-terminal Kruppel-like zinc fingers. We designate this protein as
      osteoclast-derived zinc finger (OCZF). OCZF was found to be rat homologue of
      mouse leukemia/lymphoma-related factor (LRF). Northern blot and in situ
      hybridization analysis showed OCZF mRNA at a high level in osteoclasts and kidney
      cells. OCZF had a nuclear targeting sequence and was localized in the nucleus of 
      transfected cells. In addition, OCZF specifically bound to the guanine-rich
      consensus sequences of Egr-1 and c-Krox. Transient transfection assays indicate
      that OCZF can repress transcription activity like other POZ domain proteins.
      Furthermore, antisense but not sense phosphorothioate oligodeoxynucleotides
      (ODNs) for OCZF cDNA suppressed the formation of osteoclast-like multinucleated
      cells (MNCs) in bone marrow culture, whereas the same ODNs did not significantly 
      affect the formation of macrophage polykaryons and mononuclear preosteoclast-like
      cells (POCs). These results suggest that OCZF is a unique transcription factor
      that plays an important role in the late stage of osteoclastogenesis.
FAU - Kukita, A
AU  - Kukita A
AD  - Department of Microbiology, Department of Biochemistry, Saga Medical School,
      Saga, Japan. Fukuoka, Japan. kukita@smsnet.saga-med.ac.jp
FAU - Kukita, T
AU  - Kukita T
FAU - Ouchida, M
AU  - Ouchida M
FAU - Maeda, H
AU  - Maeda H
FAU - Yatsuki, H
AU  - Yatsuki H
FAU - Kohashi, O
AU  - Kohashi O
LA  - eng
SI  - GENBANK/D88450
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ZBTB7A protein, human)
RN  - 0 (Zbtb7a protein, mouse)
RN  - 0 (Zbtb7a protein, rat)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - DNA-Binding Proteins/chemistry/genetics
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Osteoblasts/cytology
MH  - Osteoclasts/*cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Repressor Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/chemistry/*genetics/*metabolism
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
MH  - Zinc Fingers
EDAT- 1999/09/09 00:00
MHDA- 1999/09/09 00:01
CRDT- 1999/09/09 00:00
PHST- 1999/09/09 00:00 [pubmed]
PHST- 1999/09/09 00:01 [medline]
PHST- 1999/09/09 00:00 [entrez]
PST - ppublish
SO  - Blood. 1999 Sep 15;94(6):1987-97.