PMID- 10477558
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 190
IP  - 5
DP  - 1999 Sep 6
TI  - N-formylpeptides induce two distinct concentration optima for mouse neutrophil
      chemotaxis by differential interaction with two N-formylpeptide receptor (FPR)
      subtypes. Molecular characterization of FPR2, a second mouse neutrophil FPR.
PG  - 741-7
AB  - The N-formylpeptide receptor (FPR) is a G protein-coupled receptor that mediates 
      mammalian phagocyte chemotactic responses to bacterial N-formylpeptides. Here we 
      show that a mouse gene named Fpr-rs2 encodes a second N-formylpeptide receptor
      subtype selective for neutrophils which we have provisionally named FPR2. The
      prototype N-formylpeptide fMLF induced calcium flux and chemotaxis in human
      embryonic kidney (HEK) 293 cells stably transfected with FPR2. The EC(50)s,
      approximately 5 microM for calcium flux and chemotaxis, were approximately
      100-fold greater than the corresponding values for mouse FPR-transfected HEK 293 
      cells. Consistent with this, fMLF induced two distinct concentration optima for
      chemotaxis of normal mouse neutrophils, but only the high concentration optimum
      for chemotaxis of neutrophils from FPR knockout mice. Based on these data, we
      hypothesize that high- and low-affinity N-formylpeptide receptors, FPR and FPR2, 
      respectively, may function in vivo as a relay mediating neutrophil migration
      through the high and low concentration portions of N-formylpeptide gradients.
FAU - Hartt, J K
AU  - Hartt JK
AD  - Laboratory of Host Defenses, National Institute of Allergy and Infectious
      Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
FAU - Barish, G
AU  - Barish G
FAU - Murphy, P M
AU  - Murphy PM
FAU - Gao, J L
AU  - Gao JL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Peptide)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Chemotaxis, Leukocyte/*drug effects/*physiology
MH  - DNA Primers/genetics
MH  - GTP-Binding Proteins/metabolism
MH  - Humans
MH  - Ion Transport/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*drug effects/*physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/*drug effects/genetics/*physiology
MH  - Receptors, Peptide/*drug effects/genetics/*physiology
MH  - Transfection
PMC - PMC2195614
EDAT- 1999/09/08 00:00
MHDA- 1999/09/08 00:01
CRDT- 1999/09/08 00:00
PHST- 1999/09/08 00:00 [pubmed]
PHST- 1999/09/08 00:01 [medline]
PHST- 1999/09/08 00:00 [entrez]
AID - 10.1084/jem.190.5.741 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Sep 6;190(5):741-7. doi: 10.1084/jem.190.5.741.