PMID- 10477520
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 285
IP  - 5433
DP  - 1999 Sep 3
TI  - Differential stimulation of PKC phosphorylation of potassium channels by ZIP1 and
      ZIP2.
PG  - 1565-9
AB  - Targeting of protein modification enzymes is a key biochemical step to achieve
      specific and effective posttranslational modifications. Two alternatively spliced
      ZIP1 and ZIP2 proteins are described, which bind to both Kvbeta2 subunits of
      potassium channel and protein kinase C (PKC) zeta, thereby acting as a physical
      link in the assembly of PKCzeta-ZIP-potassium channel complexes. ZIP1 and ZIP2
      differentially stimulate phosphorylation of Kvbeta2 by PKCzeta. They also
      interact to form heteromultimers, which allows for a hybrid stimulatory activity 
      to PKCzeta. Finally, ZIP1 and ZIP2 coexist in the same cell type and are elevated
      differentially by neurotrophic factors. These results provide a mechanism for
      specificity and regulation of PKCzeta-targeted phosphorylation.
FAU - Gong, J
AU  - Gong J
AD  - Department of Physiology, The Johns Hopkins University School of Medicine, 725
      North Wolfe Street, Baltimore, MD 21205, USA.
FAU - Xu, J
AU  - Xu J
FAU - Bezanilla, M
AU  - Bezanilla M
FAU - van Huizen, R
AU  - van Huizen R
FAU - Derin, R
AU  - Derin R
FAU - Li, M
AU  - Li M
LA  - eng
GR  - NS33324/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Potassium Channels)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.11.1 (protein kinase C zeta)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cell Line
MH  - Cerebellum/metabolism
MH  - DNA, Complementary
MH  - Isoenzymes/metabolism
MH  - Molecular Sequence Data
MH  - Myelin Basic Protein/metabolism
MH  - Nerve Growth Factors/pharmacology
MH  - Neurons/*metabolism
MH  - Phosphorylation
MH  - Potassium Channels/*metabolism
MH  - Protein Kinase C/*metabolism
MH  - Pyramidal Cells/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Substrate Specificity
MH  - Transfection
EDAT- 1999/09/08 00:00
MHDA- 1999/09/08 00:01
CRDT- 1999/09/08 00:00
PHST- 1999/09/08 00:00 [pubmed]
PHST- 1999/09/08 00:01 [medline]
PHST- 1999/09/08 00:00 [entrez]
AID - 7789 [pii]
AID - 10.1126/science.285.5433.1565 [doi]
PST - ppublish
SO  - Science. 1999 Sep 3;285(5433):1565-9. doi: 10.1126/science.285.5433.1565.