PMID- 10473617
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 37
DP  - 1999 Sep 10
TI  - Characterization of serine 916 as an in vivo autophosphorylation site for protein
      kinase D/Protein kinase Cmu.
PG  - 26543-9
AB  - Activation of the serine kinase protein kinase D (PKD)/PKCmicro is controlled by 
      the phosphorylation of two serine residues within its activation loop via a
      PKC-dependent signaling cascade. In this study we have identified the C-terminal 
      serine 916 residue as an in vivo phosphorylation site within active PKD/PKCmu. An
      antibody that recognized PKD/PKCmu proteins specifically phosphorylated on the
      serine 916 residue was generated and used to show that phosphorylation of Ser-916
      is induced by phorbol ester treatment of cells. Thus, the pS916 antibody is a
      useful tool to study the regulation of PKD/PKCmu activity in vivo. Antigen
      receptor ligation of T and B lymphocytes also induced phosphorylation of the
      serine 916 residue of PKD/PKCmu. Furthermore the regulatory FcgammaRIIB receptor,
      which mediates vital negative feedback signals to the B cell antigen receptor
      complex, inhibited the antigen receptor-induced activation and serine 916
      phosphorylation of PKD/PKCmu. The degree of serine 916 phosphorylation during
      lymphocyte activation and inhibition exactly correlated with the activation
      status of PKD/PKCmu. Moreover, using different mutants of PKD/PKCmu, we show that
      serine 916 is not trans-phosphorylated by an upstream kinase but is rather an
      autophosphorylation event that occurs following activation of PKD/PKCmu.
FAU - Matthews, S A
AU  - Matthews SA
AD  - Lymphocyte Activation Laboratory, Imperial Cancer Research Fund, Lincoln's Inn
      Fields, London WC2A 3PX, United Kingdom.
FAU - Rozengurt, E
AU  - Rozengurt E
FAU - Cantrell, D
AU  - Cantrell D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (Phosphopeptides)
RN  - 0 (Recombinant Proteins)
RN  - 37558-16-0 (Phorbol 12,13-Dibutyrate)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.10.- (protein kinase D)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - B-Lymphocytes/enzymology
MH  - Base Sequence
MH  - COS Cells
MH  - DNA Primers
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutagenesis, Site-Directed
MH  - Peptide Mapping
MH  - Phorbol 12,13-Dibutyrate/pharmacology
MH  - Phosphopeptides/chemistry
MH  - Phosphorylation
MH  - Protein Kinase C/chemistry/genetics/*metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Serine/*metabolism
MH  - Substrate Specificity
MH  - Tumor Cells, Cultured
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - 10.1074/jbc.274.37.26543 [doi]
AID - S0021-9258(19)55247-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 10;274(37):26543-9. doi: 10.1074/jbc.274.37.26543.