PMID- 10473598
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 37
DP  - 1999 Sep 10
TI  - Characterization of the regulatory domains of the human skn-1a/Epoc-1/Oct-11 POU 
      transcription factor.
PG  - 26399-406
AB  - The Skn-1a POU transcription factor is primarily expressed in keratinocytes of
      murine embryonic and adult epidermis. Although some POU factors expressed in a
      tissue-specific manner are important for normal differentiation, the biological
      function of Skn-1a remains unknown. Previous in vitro studies indicate that
      Skn-1a has the ability to transactivate markers of keratinocyte differentiation. 
      In this study, we have characterized Skn-1a's transactivation domain(s) and
      engineered a dominant negative protein that lacked this transactivation domain.
      Deletional analysis of the human homologue of Skn-1a with three target promoters 
      revealed the presence of two functional domains: a primary C-terminal
      transactivation domain and a combined N-terminal inhibitory domain and
      transactivation domain. Skn-1a lacking the C-terminal region completely lost
      transactivation ability, irrespective of the promoter tested, and was able to
      block transactivation by normal Skn-1a in competition assays. Compared with
      full-length, Skn-1a lacking the N-terminal region demonstrated either increased
      transactivation (bovine cytokeratin 6 promoter), comparable transactivation
      (human papillomavirus type 1a long control region), or loss of transactivation
      (human papillomavirus type 18 long control region). The identification of a
      primary C-terminal transactivation domain enabled us to generate a dominant
      negative Skn-1a factor, which will be useful in the quest for a better
      understanding of this keratinocyte-specific gene regulator.
FAU - Hildesheim, J
AU  - Hildesheim J
AD  - Dermatology Branch, NCI, National Institutes of Health, Bethesda, Maryland
      20892-1908, USA.
FAU - Foster, R A
AU  - Foster RA
FAU - Chamberlin, M E
AU  - Chamberlin ME
FAU - Vogel, J C
AU  - Vogel JC
LA  - eng
SI  - GENBANK/AF162278
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11
MH  - DNA, Complementary
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Transcription Factors/*genetics
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - 10.1074/jbc.274.37.26399 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 10;274(37):26399-406. doi: 10.1074/jbc.274.37.26399.