PMID- 10473596
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 37
DP  - 1999 Sep 10
TI  - Cig30 and Pitx3 genes are arranged in a partially overlapping tail-to-tail array 
      resulting in complementary transcripts.
PG  - 26387-92
AB  - The mouse Cig30 gene codes for a 30-kDa membrane glycoprotein, which appears to
      have a role in the recruitment of brown adipose tissue. To elucidate the
      structure of the Cig30 gene, we have isolated a lambda phage genomic DNA clone
      containing the entire mouse gene and found that Cig30 consists of four exons that
      are spread over 4 kilobase pairs of genomic sequence. Using a fluorescence in
      situ hybridization assay and interspecific backcross panel mapping, we have
      localized the Cig30 locus to the distal region of mouse chromosome 19, between
      the Tlx1 and Ins1 loci. Sequencing of the corresponding lambda clone to
      completion revealed that the insert contained yet another gene in the opposite
      orientation. It turned out to be the newly identified homeobox gene Pitx3.
      Interestingly, the genes are very tightly linked, so that the 3' ends of their
      transcripts are complementary. Thus, our results provide evidence for
      bidirectional transcription of a several hundred base pair-long DNA region as a
      result of the extremely tight linkage between Cig30 and Pitx3.
FAU - Tvrdik, P
AU  - Tvrdik P
AD  - The Wenner-Gren Institute, Arrhenius Laboratories F3, Stockholm University, S-106
      91 Stockholm, Sweden.
FAU - Asadi, A
AU  - Asadi A
FAU - Kozak, L P
AU  - Kozak LP
FAU - Nuglozeh, E
AU  - Nuglozeh E
FAU - Parente, F
AU  - Parente F
FAU - Nedergaard, J
AU  - Nedergaard J
FAU - Jacobsson, A
AU  - Jacobsson A
LA  - eng
SI  - GENBANK/AF054504
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (homeobox protein PITX1)
RN  - 0 (homeobox protein PITX3)
RN  - 184787-43-7 (homeobox protein PITX2)
RN  - 9007-49-2 (DNA)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.- (Fatty Acid Elongases)
SB  - IM
MH  - Acetyltransferases
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA
MH  - Fatty Acid Elongases
MH  - Gene Expression Regulation
MH  - Genes, Overlapping
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Membrane Proteins/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Nuclear Proteins
MH  - Paired Box Transcription Factors
MH  - RNA, Messenger/*genetics
MH  - Transcription Factors/*genetics
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - 10.1074/jbc.274.37.26387 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 10;274(37):26387-92. doi: 10.1074/jbc.274.37.26387.