PMID- 10473558
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 37
DP  - 1999 Sep 10
TI  - The role of individual SH2 domains in mediating association of phospholipase
      C-gamma1 with the activated EGF receptor.
PG  - 26091-7
AB  - The two SH2 (Src homology domain 2) domains present in phospholipase C-gamma1
      (PLC-gamma1) were assayed for their capacities to recognize the five
      autophosphorylation sites in the epidermal growth factor receptor. Plasmon
      resonance and immunological techniques were employed to measure interactions
      between SH2 fusion proteins and phosphotyrosine-containing peptides. The N-SH2
      domain recognized peptides in the order of pY1173 > pY992 > pY1068 > pY1148 >>
      pY1086, while the C-SH2 domain recognized peptides in the order of pY992 > pY1068
      > pY1148 >> pY1086 and pY1173. The major autophosphorylation site, pY1173, was
      recognized only by the N-SH2 domain. Contributions of the N-SH2 and C-SH2 domains
      to the association of the intact PLC-gamma1 molecule with the activated epidermal
      growth factor (EGF) receptor were assessed in vivo. Loss of function mutants of
      each SH2 domain were produced in a full-length epitope-tagged PLC-gamma1. After
      expression of the mutants, cells were treated with EGF and association of
      exogenous PLC-gamma1 with EGF receptors was measured. In this context the N-SH2
      is the primary contributor to PLC-gamma1 association with the EGF receptor. The
      combined results suggest an association mechanism involving the N-SH2 domain and 
      the pY1173 autophosphorylation site as a primary event and the C-SH2 domain and
      the pY992 autophosphorylation site as a secondary event.
FAU - Chattopadhyay, A
AU  - Chattopadhyay A
AD  - Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, 
      Tennessee 37232-0146, USA.
FAU - Vecchi, M
AU  - Vecchi M
FAU - Ji, Q s
AU  - Ji Qs
FAU - Mernaugh, R
AU  - Mernaugh R
FAU - Carpenter, G
AU  - Carpenter G
LA  - eng
GR  - CA24071/CA/NCI NIH HHS/United States
GR  - CA68485/CA/NCI NIH HHS/United States
GR  - CA75195/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Isoenzymes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay
MH  - ErbB Receptors/*metabolism
MH  - Isoenzymes/chemistry/*metabolism
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Recombinant Fusion Proteins/metabolism
MH  - Surface Plasmon Resonance
MH  - Type C Phospholipases/chemistry/*metabolism
MH  - *src Homology Domains
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - 10.1074/jbc.274.37.26091 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 10;274(37):26091-7. doi: 10.1074/jbc.274.37.26091.