PMID- 10473346
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20191103
IS  - 1040-9238 (Print)
IS  - 1040-9238 (Linking)
VI  - 34
IP  - 3
DP  - 1999
TI  - Replication protein A (RPA): the eukaryotic SSB.
PG  - 141-80
AB  - Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding
      protein that is highly conserved in eukaryotes. RPA plays essential roles in many
      aspects of nucleic acid metabolism, including DNA replication, nucleotide
      excision repair, and homologous recombination. In this review, we provide a
      comprehensive overview of RPA structure and function and highlight the more
      recent developments in these areas. The last few years have seen major advances
      in our understanding of the mechanism of RPA binding to DNA, including the
      structural characterization of the primary DNA-binding domains (DBD) and the
      identification of two secondary DBDs. Moreover, evidence indicates that RPA
      utilizes a multistep pathway to bind single-stranded DNA involving a particular
      molecular polarity of RPA, a mechanism that is apparently used to facilitate
      origin denaturation. In addition to its mechanistic roles, RPA interacts with
      many key factors in nucleic acid metabolism, and we discuss the critical nature
      of many of these interactions to DNA metabolism. RPA is a phosphorylation target 
      for DNA-dependent protein kinase (DNA-PK) and likely the ataxia
      telangiectasia-mutated gene (ATM) protein kinase, and recent observations are
      described that suggest that RPA phosphorylation plays a significant modulatory
      role in the cellular response to DNA damage.
FAU - Iftode, C
AU  - Iftode C
AD  - Department of Biochemistry and Kaplan Comprehensive Cancer Center, New York
      University Medical Center, NY 10016, USA.
FAU - Daniely, Y
AU  - Daniely Y
FAU - Borowiec, J A
AU  - Borowiec JA
LA  - eng
GR  - AI29963/AI/NIAID NIH HHS/United States
GR  - CA62198/CA/NCI NIH HHS/United States
GR  - P30CA16087/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - Crit Rev Biochem Mol Biol
JT  - Critical reviews in biochemistry and molecular biology
JID - 8903774
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Replication Protein A)
SB  - IM
MH  - Binding Sites
MH  - DNA Repair
MH  - DNA Replication
MH  - DNA, Single-Stranded/*metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Eukaryotic Cells
MH  - Protein Binding
MH  - Recombination, Genetic
MH  - Replication Protein A
RF  - 234
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - 10.1080/10409239991209255 [doi]
PST - ppublish
SO  - Crit Rev Biochem Mol Biol. 1999;34(3):141-80. doi: 10.1080/10409239991209255.