PMID- 10471929
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20091119
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 17
IP  - 9
DP  - 1999 Sep
TI  - Genetic screens in yeast to identify mammalian nonreceptor modulators of
      G-protein signaling.
PG  - 878-83
AB  - We describe genetic screens in Saccharomyces cerevisiae designed to identify
      mammalian nonreceptor modulators of G-protein signaling pathways. Strains lacking
      a pheromone-responsive G-protein coupled receptor and expressing a
      mammalian-yeast Galpha hybrid protein were made conditional for growth upon
      either pheromone pathway activation (activator screen) or pheromone pathway
      inactivation (inhibitor screen). Mammalian cDNAs that conferred plasmid-dependent
      growth under restrictive conditions were identified. One of the cDNAs identified 
      from the activator screen, a human Ras-related G protein that we term AGS1 (for
      activator of G-protein signaling), appears to function by facilitating guanosine 
      triphosphate (GTP) exchange on the heterotrimeric Galpha. A cDNA product
      identified from the inhibitor screen encodes a previously identified regulator of
      G-protein signaling, human RGS5.
FAU - Cismowski, M J
AU  - Cismowski MJ
AD  - Cadus Pharmaceutical Corporation, 777 Old Saw Mill River Road, Tarrytown 10591,
      NY, USA.
FAU - Takesono, A
AU  - Takesono A
FAU - Ma, C
AU  - Ma C
FAU - Lizano, J S
AU  - Lizano JS
FAU - Xie, X
AU  - Xie X
FAU - Fuernkranz, H
AU  - Fuernkranz H
FAU - Lanier, S M
AU  - Lanier SM
FAU - Duzic, E
AU  - Duzic E
LA  - eng
SI  - GENBANK/AF069506
SI  - GENBANK/AF159570
GR  - R01-NS24821/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (DNA, Complementary)
RN  - 0 (Pheromones)
RN  - 0 (RASD1 protein, human)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
CIN - Nat Biotechnol. 1999 Sep;17(9):852-3. PMID: 10471921
MH  - Cloning, Molecular/*methods
MH  - DNA, Complementary/genetics
MH  - Epistasis, Genetic
MH  - GTP-Binding Proteins/genetics/*isolation & purification/*metabolism
MH  - Gene Library
MH  - Genetic Engineering
MH  - Humans
MH  - Phenotype
MH  - Pheromones/metabolism
MH  - Protein Binding
MH  - Saccharomyces cerevisiae/cytology/*genetics
MH  - Selection, Genetic
MH  - Signal Transduction/*genetics
MH  - Transformation, Genetic
MH  - ras Proteins/genetics/*isolation & purification
EDAT- 1999/09/03 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/09/03 09:00
PHST- 1999/09/03 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/09/03 09:00 [entrez]
AID - 10.1038/12867 [doi]
PST - ppublish
SO  - Nat Biotechnol. 1999 Sep;17(9):878-83. doi: 10.1038/12867.