PMID- 10471803
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 457
IP  - 3
DP  - 1999 Sep 3
TI  - The proteasome inhibitor PI31 competes with PA28 for binding to 20S proteasomes.
PG  - 333-8
AB  - PI31 is a previously described inhibitor of 20S proteasomes. Using recombinant
      PI31 we have analyzed its effect on proteasomal hydrolyzing activity of short
      fluorogenic substrates and of a synthetic 40-mer polypeptide. In addition, we
      investigated its influence on the activation of 20S proteasome by the proteasome 
      activator PA28. PI31 inhibits polypeptide degradation already at concentrations
      which only partially inhibit fluorogenic substrate turnover and immunosubunits do
      not influence the PI31 binding affinity. Furthermore our data demonstrate that
      PI31 is a potent competitor of PA28-mediated activation.
FAU - Zaiss, D M
AU  - Zaiss DM
AD  - Institute of Biochemistry/Charite, Monbijou Strasse 2, D-10117, Berlin, Germany.
FAU - Standera, S
AU  - Standera S
FAU - Holzhutter, H
AU  - Holzhutter H
FAU - Kloetzel, P
AU  - Kloetzel P
FAU - Sijts, A J
AU  - Sijts AJ
LA  - eng
SI  - GENBANK/D88378
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Autoantigens)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Ki antigen)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Muscle Proteins)
RN  - 0 (PSME1 protein, human)
RN  - 0 (PSMF1 protein, human)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoantigens
MH  - Binding, Competitive
MH  - Cell Line/drug effects/metabolism
MH  - Cloning, Molecular
MH  - Cysteine Endopeptidases/drug effects/*metabolism
MH  - Cysteine Proteinase Inhibitors/*genetics/*metabolism/pharmacology
MH  - Enzyme Activation
MH  - Glutathione Transferase/genetics/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism/pharmacology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/drug effects/*metabolism
MH  - *Muscle Proteins
MH  - Proteasome Endopeptidase Complex
MH  - Proteins/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - S0014579399010728 [pii]
AID - 10.1016/s0014-5793(99)01072-8 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Sep 3;457(3):333-8. doi: 10.1016/s0014-5793(99)01072-8.