PMID- 10471800
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 457
IP  - 3
DP  - 1999 Sep 3
TI  - Oligomeric complexes involved in translocation of proteins across the membrane of
      the endoplasmic reticulum.
PG  - 316-22
AB  - Proteins involved in protein translocation across the membrane of the endoplasmic
      reticulum assemble into different oligomeric complexes depending on their state
      of function. To analyse such membrane protein complexes we fractionated proteins 
      of mammalian rough microsomes and analysed them using blue native PAGE and
      immunoblotting. Among the proteins characterised are the Sec61p complex, the
      oligosaccharyl transferase (OST) complex, the translocon-associated protein
      (TRAP) complex, the TRAM and RAMP4 proteins, the signal recognition particle
      (SRP) and the SRP receptor (SR). Interestingly, the RAMP4 protein, SR and OST
      complex display more than one oligomeric form.
FAU - Wang, L
AU  - Wang L
AD  - Zentrum fur Molekulare Biologie der Universitat Heidelberg (ZMBH), Im Neuenheimer
      Feld 282, D-69120, Heidelberg, Germany.
FAU - Dobberstein, B
AU  - Dobberstein B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calreticulin)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Peptide)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SEC Translocation Channels)
RN  - 0 (endoplasmic reticulum glycoprotein p72)
RN  - 0 (ribophorin)
RN  - 0 (signal peptide receptor)
RN  - 0 (signal sequence receptor)
RN  - EC 5.3.4.1 (Protein Disulfide-Isomerases)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Calcium-Binding Proteins/metabolism
MH  - Calreticulin
MH  - Dogs
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Endoplasmic Reticulum, Rough/*metabolism
MH  - Immunoblotting
MH  - Intracellular Membranes/*metabolism
MH  - Membrane Glycoproteins/isolation & purification/metabolism
MH  - Membrane Proteins/immunology/isolation & purification/metabolism
MH  - Protein Disulfide-Isomerases/metabolism
MH  - Proteins/immunology/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/chemistry/*metabolism
MH  - Receptors, Peptide/chemistry/*metabolism
MH  - Ribonucleoproteins/metabolism
MH  - Ribosomes/metabolism
MH  - SEC Translocation Channels
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - S0014-5793(99)01075-3 [pii]
AID - 10.1016/s0014-5793(99)01075-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Sep 3;457(3):316-22. doi: 10.1016/s0014-5793(99)01075-3.