PMID- 10471791
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 457
IP  - 2
DP  - 1999 Aug 27
TI  - CA-MMP: a matrix metalloproteinase with a novel cysteine array, but without the
      classic cysteine switch.
PG  - 262-70
AB  - A matrix metalloproteinase (MMP)-like gene was identified in mouse to contain a
      conserved MMP catalytic domain and an RRRR motif. It lacks a classic cysteine
      switch, but it possesses two novel motifs: a cysteine array
      (Cys-X(6)-Cys-X(8)-Cys-X(10)-Cys-X(3)-Cys-X(2)-Cys), and a novel Ig-fold. It is
      named CA-MMP after the distinct cysteine array motif, and little is known about
      its biochemical function. In an attempt to characterize CA-MMP activity, the
      full-length sequence was expressed in mammalian cells and its product found to be
      cell-associated without detectable secretion. In light of this unusual finding, a
      chimera combining the catalytic domain of CA-MMP with the prodomain of
      stromelysin-3 was constructed to express a fully active enzyme in mammalian
      cells. Purified CA-MMP catalytic domain expresses proteolytic activity against
      protein substrates in an MMP inhibitor sensitive fashion. Taken together, it is
      concluded that CA-MMP is an MMP with distinct structure, biochemical properties
      and evolutionary history that may define a new subclass of the MMP superfamily.
FAU - Pei, D
AU  - Pei D
AD  - Department of Pharmacology, 3-249 Millard Hall, 435 Delaware St. S.E. ,University
      of Minnesota, Minneapolis, MN, USA. peixx003@tc.umn.edu
LA  - eng
SI  - GENBANK/AF085742
GR  - CA76308/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - EC 3.4.24.- (CA-matrix metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.65 (Matrix Metalloproteinase 12)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Cysteine/*chemistry
MH  - DNA, Complementary/analysis
MH  - Embryo, Mammalian/enzymology
MH  - Exons
MH  - Extracellular Matrix/enzymology
MH  - Introns
MH  - Matrix Metalloproteinase 12
MH  - *Matrix Metalloproteinases
MH  - Metalloendopeptidases/biosynthesis/chemistry/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - S0014-5793(99)01046-7 [pii]
AID - 10.1016/s0014-5793(99)01046-7 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Aug 27;457(2):262-70. doi: 10.1016/s0014-5793(99)01046-7.