PMID- 10471790
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 457
IP  - 2
DP  - 1999 Aug 27
TI  - Involvement of branched-chain amino acid aminotransferase (Bcat1/Eca39) in
      apoptosis.
PG  - 255-61
AB  - The branched-chain amino acid aminotransferase, Bcat1/Eca39, catalyzes the first 
      step of branched-chain amino acid catabolism. Bcat1/Eca39 was originally isolated
      from a c-myc-induced tumor and was proven to be a direct target for c-Myc
      regulation. The gene is highly conserved in evolution and disruption of its yeast
      homolog affects cell growth. To assess the role of Bcat1/Eca39 in mammalian
      cells, we overexpressed Bcat1/Eca39 in murine cells and studied effects on cell
      growth. Overexpression of Bcat1/Eca39 had no apparent effect on the proliferation
      of cells grown with high serum concentrations, but under serum deprivation
      conditions, led to a decrease in cell viability. Cell death under these
      conditions displayed apoptotic features. The branched-chain keto acid,
      alpha-ketoisocaproate, a metabolite of leucine catabolism produced by
      BCAT1/ECA39, was previously found to inhibit cell growth. We show that
      alpha-ketoisocaproate can induce rapid apoptotic cell death. This observation
      suggests that the growth inhibitory effect of BCAT1/ECA39 and its apoptosis
      promoting effect may be mediated by the levels of the products of BCAT1/ECA39
      activity, namely, branched-chain keto acids.
FAU - Eden, A
AU  - Eden A
AD  - Department of Genetics, Institute of Life Sciences, The Hebrew University of
      Jerusalem, Givat-Ram, Jerusalem, Israel.
FAU - Benvenisty, N
AU  - Benvenisty N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Keto Acids)
RN  - 0 (Proteins)
RN  - 816-66-0 (alpha-ketoisocaproic acid)
RN  - EC 2.6.1. (Bcat1 protein, mouse)
RN  - EC 2.6.1.- (Transaminases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Cell Division/physiology
MH  - Keto Acids/pharmacology
MH  - Mice
MH  - Plasmids/genetics
MH  - Protein Biosynthesis
MH  - Proteins/*physiology
MH  - *Transaminases
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - S0014-5793(99)01054-6 [pii]
AID - 10.1016/s0014-5793(99)01054-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Aug 27;457(2):255-61. doi: 10.1016/s0014-5793(99)01054-6.