PMID- 10471789
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 457
IP  - 2
DP  - 1999 Aug 27
TI  - Association of the rat heterogeneous nuclear RNA-ribonucleoprotein F with
      TATA-binding protein.
PG  - 251-4
AB  - Heterogeneous nuclear ribonucleoprotein F (hnRNP-F) has been shown to be a
      pre-mRNA splicing factor. Recent studies have uncovered the coordination of
      synthesis of pre-mRNA and its processing, including post-transcriptional
      modification and splicing. Here, we present evidence for an association between a
      splicing factor, hnRNP-F, and TATA-binding protein (TBP), which is an essential
      factor needed for transcription initiation. An affinity detection experiment
      revealed hnRNP-F in the preparation of TBP-interacting proteins. HnRNP-F was
      associated with TBP in nuclear extracts and was capable of direct binding to TBP 
      in vitro. These results suggest that hnRNP-F is associated with TBP in the cell. 
      HnRNP-F was observed in abundance in the thymus, spleen and testis, and its
      distribution pattern was similar to that of TBP, implying a functional
      coordination of transcription and splicing. We assume that the splicing machinery
      is associated with the transcription apparatus as a prerequisite prior to
      transcriptional elongation.
FAU - Yoshida, T
AU  - Yoshida T
AD  - Department of Biology, Faculty of Science, Chiba University, Chiba, Japan.
FAU - Makino, Y
AU  - Makino Y
FAU - Tamura, T
AU  - Tamura T
LA  - eng
SI  - GENBANK/AB022209
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein Group F-H)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (RNA, Nuclear)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/*metabolism
MH  - Heterogeneous-Nuclear Ribonucleoprotein Group F-H
MH  - Heterogeneous-Nuclear Ribonucleoproteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Nuclear/*metabolism
MH  - Rats
MH  - Ribonucleoproteins/genetics/*metabolism
MH  - TATA-Box Binding Protein
MH  - Transcription Factors/*metabolism
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - S0014579399010480 [pii]
AID - 10.1016/s0014-5793(99)01048-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Aug 27;457(2):251-4. doi: 10.1016/s0014-5793(99)01048-0.