PMID- 10471747
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 18
DP  - 1999 Sep 15
TI  - Drosophila and human RecQ5 exist in different isoforms generated by alternative
      splicing.
PG  - 3762-9
AB  - Members of the RecQ helicase superfamily have been implicated in DNA repair,
      recombination and replication. Although the genome of the budding yeast
      Saccharomyces cerevisiae encodes only a single member of this family, there are
      at least five human RecQ-related genes: RecQL, BLM, WRN, RecQ4 and RecQ5.
      Mutations in at least three of these are associated with diseases involving a
      predisposition to malignancies and a cellular phenotype that includes increased
      chromosome instability. Metazoan RecQ helicases are defined by a core region with
      characteristic helicase motifs and sequence similarity to Escherichia coli RecQ
      protein. This core region is typically flanked by extensive, highly charged
      regions, of largely unknown function. The recently reported human RecQ5, however,
      has only the core RecQ-homologous region. We describe here the identification of 
      the Drosophila RecQ5 gene. We recovered cDNAs corresponding to three alternative 
      splice forms of the RecQ5 transcript. Two of these generate nearly identical 54
      kDa proteins that, like human RecQ5, consist of the helicase core only. The third
      splice variant encodes a 121 kDa isoform that, like other family members, has a
      C-terminal extension rich in charged residues. A combination of RACE and cDNA
      analysis of human RECQ5 demonstrates extensive alternative splicing for this gene
      also, including some forms lacking helicase motifs and other conserved regions.
FAU - Sekelsky, J J
AU  - Sekelsky JJ
AD  - Section of MCB, University of California, Davis, CA 95616, USA.
      sekelsky@email.unc.edu
FAU - Brodsky, M H
AU  - Brodsky MH
FAU - Rubin, G M
AU  - Rubin GM
FAU - Hawley, R S
AU  - Hawley RS
LA  - eng
SI  - GENBANK/AF134239
SI  - GENBANK/AF135183
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Insect Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RECQL5 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (RecQ Helicases)
SB  - IM
MH  - Alternative Splicing/*genetics
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Nucleus/metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Conserved Sequence/genetics
MH  - DNA Helicases/chemistry/*genetics/metabolism
MH  - Drosophila melanogaster/cytology/*genetics
MH  - Expressed Sequence Tags
MH  - Humans
MH  - Insect Proteins/chemistry/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phylogeny
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - RNA, Messenger/analysis/genetics
MH  - RecQ Helicases
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
PMC - PMC148633
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - gkc555 [pii]
AID - 10.1093/nar/27.18.3762 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Sep 15;27(18):3762-9. doi: 10.1093/nar/27.18.3762.