PMID- 10471742
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 18
DP  - 1999 Sep 15
TI  - Identification of a mammalian RNA polymerase I holoenzyme containing components
      of the DNA repair/replication system.
PG  - 3720-7
AB  - Traditional models for transcription initiation by RNA polymerase I include a
      stepwise assembly of basic transcription factors/regulatory proteins on the core 
      promoter to form a preinitiation complex. In contrast, we have identified a
      preassembled RNA polymerase I (RPI) complex that contains all the factors
      necessary and sufficient to initiate transcription from the rDNA promoter in
      vitro. The purified RPI holoenzyme contains the RPI homolog of TFIID, SL-1 and
      the rDNA transcription terminator factor (TTF-1), but lacks UBF, an activator of 
      rDNA transcription. Certain components of the DNA repair/replication system,
      including Ku70/80, DNA topoisomerase I and PCNA, are also associated with the RPI
      complex. We have found that the holo-enzyme supported specific transcription and 
      that specific transcription was stimulated by the RPI transcription activator
      UBF. These results support the hypothesis that a fraction of the RPI exists as a 
      preassembled, transcriptionally competent complex that is readily recruited to
      the rDNA promoter, i.e. as a holoenzyme, and provide important new insights into 
      the mechanisms governing initiation by RPI.
FAU - Hannan, R D
AU  - Hannan RD
AD  - Cancer Research Centre and Department of Biochemistry, Laval University,
      Hotel-Dieu de Quebec, 11 Cote du Palais, Quebec G1R 2J6, Canada.
FAU - Cavanaugh, A
AU  - Cavanaugh A
FAU - Hempel, W M
AU  - Hempel WM
FAU - Moss, T
AU  - Moss T
FAU - Rothblum, L
AU  - Rothblum L
LA  - eng
GR  - GM46991/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Antigens, Nuclear)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Holoenzymes)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Pol1 Transcription Initiation Complex Proteins)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (transcription factor UBF)
RN  - 0 (transcription initiation factor TIF-IB)
RN  - EC 2.7.7.6 (RNA Polymerase I)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (XRCC5 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, rat)
RN  - EC 4.2.99.- (Ku Autoantigen)
RN  - EC 5.99.1.2 (DNA Topoisomerases, Type I)
SB  - IM
MH  - Animals
MH  - *Antigens, Nuclear
MH  - *DNA Helicases
MH  - *DNA Repair/genetics
MH  - *DNA Replication/genetics
MH  - DNA Topoisomerases, Type I/isolation & purification/metabolism
MH  - DNA, Ribosomal/genetics
MH  - DNA-Binding Proteins/analysis/isolation & purification/metabolism/pharmacology
MH  - Holoenzymes/chemistry/isolation & purification/metabolism
MH  - Ku Autoantigen
MH  - Molecular Weight
MH  - Multienzyme Complexes/*chemistry/isolation & purification/metabolism
MH  - Nuclear Proteins/isolation & purification/metabolism
MH  - *Pol1 Transcription Initiation Complex Proteins
MH  - Proliferating Cell Nuclear Antigen/isolation & purification/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - RNA Polymerase I/*chemistry/*isolation & purification/metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/chemistry/isolation & purification/metabolism
MH  - Transcription Factors/analysis/chemistry/*isolation &
      purification/metabolism/pharmacology
MH  - Transcription, Genetic/drug effects/genetics
MH  - Tumor Cells, Cultured
PMC - PMC148628
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - gkc549 [pii]
AID - 10.1093/nar/27.18.3720 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Sep 15;27(18):3720-7. doi: 10.1093/nar/27.18.3720.