PMID- 10471737
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 18
DP  - 1999 Sep 15
TI  - Messenger RNAs encoding mouse histone macroH2A1 isoforms are expressed at similar
      levels in male and female cells and result from alternative splicing.
PG  - 3685-9
AB  - Two protein isoforms of histone macroH2A1 (mH2A1) are found in mammalian cells.
      One isoform, mH2A1.2 is highly concentrated on the heterochromatinized inactive X
      chromosome (Xi) of female cells. mH2A1.2 protein is also present in male cells,
      but fails to form dense concentrations. Another protein isoform, mH2A1.1, differs
      from mH2A1.2 by a single short segment of amino acids. In this study, we cloned
      and characterized the genomic locus of the mouse mH2A1 gene and mapped it to
      chromosome 13. Two alternatively spliced transcripts derived from the mH2A1 locus
      are responsible for the generation of the two mH2A1 protein isoforms with mH2A1.2
      mRNA being the most abundant spliced form in all tissues examined. The absolute
      amount of mH2A1 mRNA is similar in male and female cells for most tissues with
      the exception of testes where it is par-ticularly abundant. Both spliced forms
      are present in all adult tissues analyzed as well as in female embryonic stem
      cells. In contrast, male embryonic stem cells expressed mH2A1.1 at low levels if 
      at all. The relatively abundant expression of mH2A1 in both sexes suggests that
      mH2A1 has functions in addition to a possible involvement in X chromosome
      inactivation.
FAU - Rasmussen, T P
AU  - Rasmussen TP
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
FAU - Huang, T
AU  - Huang T
FAU - Mastrangelo, M A
AU  - Mastrangelo MA
FAU - Loring, J
AU  - Loring J
FAU - Panning, B
AU  - Panning B
FAU - Jaenisch, R
AU  - Jaenisch R
LA  - eng
SI  - GENBANK/AF171080
SI  - GENBANK/AF171081
GR  - 5R35CA44339/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Histones)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (macroH2A histone)
SB  - IM
MH  - Aging
MH  - Alternative Splicing/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Differentiation
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Dosage Compensation, Genetic
MH  - Exons/genetics
MH  - Expressed Sequence Tags
MH  - Female
MH  - Histones/chemistry/*genetics/physiology
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Protein Isoforms/genetics
MH  - RNA, Messenger/analysis/*genetics
MH  - Stem Cells/cytology/metabolism
MH  - Testis/metabolism
PMC - PMC148623
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - gkc540 [pii]
AID - 10.1093/nar/27.18.3685 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Sep 15;27(18):3685-9. doi: 10.1093/nar/27.18.3685.