PMID- 10471500
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20131121
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 23
IP  - 1
DP  - 1999 Sep
TI  - Mi-2 complex couples DNA methylation to chromatin remodelling and histone
      deacetylation.
PG  - 62-6
AB  - Methylation of DNA at the dinucleotide CpG is essential for mammalian development
      and is correlated with stable transcriptional silencing. This transcriptional
      silencing has recently been linked at a molecular level to histone deacetylation 
      through the demonstration of a physical association between histone deacetylases 
      and the methyl CpG-binding protein MeCP2 (refs 4,5). We previously purified a
      histone deacetylase complex from Xenopus laevis egg extracts that consists of six
      subunits, including an Rpd3-like deacetylase, the RbA p48/p46 histone-binding
      protein and the nucleosome-stimulated ATPase Mi-2 (ref. 6). Similar species were 
      subsequently isolated from human cell lines, implying functional conservation
      across evolution. This complex represents the most abundant form of deacetylase
      in amphibian eggs and cultured mammalian cells. Here we identify the remaining
      three subunits of this enzyme complex. One of them binds specifically to
      methylated DNA in vitro and molecular cloning reveals a similarity to a known
      methyl CpG-binding protein. Our data substantiate the mechanistic link between
      DNA methylation, histone deacetylation and transcriptional silencing.
FAU - Wade, P A
AU  - Wade PA
AD  - Laboratory of Molecular Embryology, National Institute of Child Health and Human 
      Development, National Institutes of Health, Building 18T Room 106, Bethesda,
      Maryland 20892, USA.
FAU - Gegonne, A
AU  - Gegonne A
FAU - Jones, P L
AU  - Jones PL
FAU - Ballestar, E
AU  - Ballestar E
FAU - Aubry, F
AU  - Aubry F
FAU - Wolffe, A P
AU  - Wolffe AP
LA  - eng
SI  - GENBANK/AF171099
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (Autoantigens)
RN  - 0 (CHD4 protein, human)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.5.1.98 (Mi-2 Nucleosome Remodeling and Deacetylase Complex)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
CIN - Nat Genet. 1999 Sep;23(1):5-6. PMID: 10471484
MH  - *Adenosine Triphosphatases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoantigens/metabolism/*physiology
MH  - Cell Line
MH  - Chromatin/*metabolism
MH  - *DNA Helicases
MH  - *DNA Methylation
MH  - DNA, Complementary/analysis
MH  - DNA-Binding Proteins/metabolism
MH  - Expressed Sequence Tags
MH  - Gene Library
MH  - Histone Deacetylases/metabolism
MH  - Histones/*metabolism
MH  - Humans
MH  - Mi-2 Nucleosome Remodeling and Deacetylase Complex
MH  - Mice
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Xenopus/embryology
MH  - Zinc Fingers/physiology
EDAT- 1999/09/02 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/09/02 09:00
PHST- 1999/09/02 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/09/02 09:00 [entrez]
AID - 10.1038/12664 [doi]
PST - ppublish
SO  - Nat Genet. 1999 Sep;23(1):62-6. doi: 10.1038/12664.