PMID- 10471399
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 3
DP  - 1999 Sep 7
TI  - Human placental sodium-dependent vitamin C transporter (SVCT2): molecular cloning
      and transport function.
PG  - 762-8
AB  - We report here on the cloning and functional characterization of human SVCT2, a
      sodium-dependent vitamin C (ascorbate) transporter. The hSVCT2 cDNA obtained from
      a human placental choriocarcinoma cell cDNA library, codes for a protein of 650
      amino acids with a predicted molecular mass of 70 kDa. At the level of amino acid
      sequence, the human SVCT2 exhibits 95% identity to its rat homolog. When
      functionally expressed in mammalian cells, hSVCT2 induces the transport of
      ascorbic acid. The transport process induced by hSVCT2 is Na(+)-dependent and is 
      specific for ascorbate. The Michaelis-Menton constant (K(t)) for the transport of
      ascorbate in cDNA-transfected cells is 69 +/- 5 microM. The relationship between 
      the cDNA-specific uptake rate of ascorbate and Na(+) concentration is sigmoidal
      with a Na(+):ascorbate stoichiometry of 2:1. Northern blot analysis shows that
      SVCT2-specific transcripts are present in heart, brain, placenta, and liver and
      is absent in lung and skeletal muscle. The size of the principal transcript is
      approximately 7.5 kb.
CI  - Copyright 1999 Academic Press.
FAU - Rajan, D P
AU  - Rajan DP
AD  - Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta,
      Georgia, 30912, USA.
FAU - Huang, W
AU  - Huang W
FAU - Dutta, B
AU  - Dutta B
FAU - Devoe, L D
AU  - Devoe LD
FAU - Leibach, F H
AU  - Leibach FH
FAU - Ganapathy, V
AU  - Ganapathy V
FAU - Prasad, P D
AU  - Prasad PD
LA  - eng
SI  - GENBANK/AF164142
GR  - HD33347/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Organic Anion Transporters, Sodium-Dependent)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SLC23A2 protein, human)
RN  - 0 (Slc23a2 protein, rat)
RN  - 0 (Sodium-Coupled Vitamin C Transporters)
RN  - 0 (Symporters)
RN  - 9NEZ333N27 (Sodium)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Ascorbic Acid/metabolism
MH  - Base Sequence
MH  - Biological Transport
MH  - Cell Line
MH  - Choriocarcinoma
MH  - Cloning, Molecular
MH  - Female
MH  - Gene Library
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - *Organic Anion Transporters, Sodium-Dependent
MH  - Pregnancy
MH  - Proteins/*genetics/*metabolism
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Sodium/metabolism
MH  - Sodium-Coupled Vitamin C Transporters
MH  - Substrate Specificity
MH  - *Symporters
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Uterine Neoplasms
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - 10.1006/bbrc.1999.1272 [doi]
AID - S0006-291X(99)91272-X [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Sep 7;262(3):762-8. doi: 10.1006/bbrc.1999.1272.