PMID- 10471324
OWN - NLM
STAT- MEDLINE
DCOM- 19991005
LR  - 20071114
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 251
IP  - 2
DP  - 1999 Sep 15
TI  - Identification of (CAG)(n) and (CGG)(n) repeat-binding proteins, CAGERs expressed
      in mature neurons of the mouse brain.
PG  - 388-400
AB  - The trinucleotide repeats (CAG)(n) and (CGG)(n) have been shown to be expanded in
      responsible genes of several human hereditary neurological disorders. In studies 
      of mice, we previously identified two homologous single-stranded (ss)(CAG) and
      ss(CGG) repeat-binding proteins, CAGER-1 (44 kDa) and CAGER-2 (40 kDa)
      (CAG-element-recognizing proteins). The specific binding activities of these
      proteins were predominantly detected in the mouse brain. We have isolated the
      cDNAs encoding CAGER-1 and CAGER-2 and found that they were identical to
      previously reported cDNAs for Puralpha and Purbeta, respectively. Puralpha of 28 
      kDa was previously identified as a replication-origin-binding protein that is
      ubiquitously expressed in proliferating cells. We show that the transcripts of
      CAGERs increase after birth and are detected at high levels in the adult mouse
      brain but at very low or virtually undetectable levels in other mouse tissues.
      Biochemical properties and molecular weights are different between CAGERs and
      Puralpha/beta. Immunostaining with specific antibodies against CAGERs indicates
      that CAGERs in the mouse brain reside in nonproliferating neurons but not in
      proliferating glia. We conclude that CAGERs and Puralpha/beta are unrelated
      proteins, and CAGERs are neuronal single-stranded sequence-binding proteins in
      the mouse brain. Misassignment of cDNAs is described.
CI  - Copyright 1999 Academic Press.
FAU - Yano, H
AU  - Yano H
AD  - Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
FAU - Wang, B E
AU  - Wang BE
FAU - Ahmad, I
AU  - Ahmad I
FAU - Zhang, J
AU  - Zhang J
FAU - Abo, T
AU  - Abo T
FAU - Nakayama, J
AU  - Nakayama J
FAU - Krempen, K
AU  - Krempen K
FAU - Kohwi, Y
AU  - Kohwi Y
LA  - eng
GR  - R01CA51377/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Pura protein, mouse)
RN  - 0 (Purb protein, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Brain Chemistry/*genetics
MH  - Cyclic AMP Response Element-Binding Protein/genetics/isolation &
      purification/metabolism
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/genetics/isolation & purification/metabolism
MH  - Gene Library
MH  - Hippocampus/chemistry/cytology
MH  - Mice
MH  - Nerve Tissue Proteins/genetics/isolation & purification/*metabolism
MH  - Neuroglia/chemistry
MH  - Neurons/*chemistry
MH  - Protein Binding
MH  - RNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Tissue Distribution
MH  - Transcription Factors
MH  - *Trinucleotide Repeats
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - 10.1006/excr.1999.4603 [doi]
AID - S0014-4827(99)94603-7 [pii]
PST - ppublish
SO  - Exp Cell Res. 1999 Sep 15;251(2):388-400. doi: 10.1006/excr.1999.4603.