PMID- 10471110
OWN - NLM
STAT- MEDLINE
DCOM- 20000124
LR  - 20190915
IS  - 0952-3278 (Print)
IS  - 0952-3278 (Linking)
VI  - 60
IP  - 5-6
DP  - 1999 May-Jun
TI  - Molecular aspects of fatty acid transport: mutations in the IYTSGTTGXPK motif
      impair fatty acid transport protein function.
PG  - 285-9
AB  - The murine fatty acid transport protein (FATP) facilitates uptake of long chain
      fatty acids (LCFAs) when expressed in mammalian cells. FATP's sequence contains a
      highly conserved motif, IYTSGTTGXPK, also found in a number of proteins known to 
      interact with ATP. To explore the role of this motif, we independently mutated
      the central serine (serine 250) and threonine (threonine 252) residues in this
      motif and assessed the effects of these mutations on FATP function. When
      expressed in fibroblasts, the FATP mutants demonstrated impaired LCFA import and 
      impaired binding of [alpha-32P]8-azido-ATP (azido-ATP) compared with wild-type
      FATP. These results suggest that serine 250 and threonine 252 are critical for
      FATP function and that the mechanism of action of FATP involves nucleotide
      binding which is dependent on these residues.
FAU - Stuhlsatz-Krouper, S M
AU  - Stuhlsatz-Krouper SM
AD  - Center for Cardiovascular Research, Department of Internal Medicine, Washington
      University School of Medicine, St. Louis, MO 63110-1010, USA.
FAU - Bennett, N E
AU  - Bennett NE
FAU - Schaffer, J E
AU  - Schaffer JE
LA  - eng
PT  - Journal Article
PL  - Scotland
TA  - Prostaglandins Leukot Essent Fatty Acids
JT  - Prostaglandins, leukotrienes, and essential fatty acids
JID - 8802730
RN  - 0 (Carrier Proteins)
RN  - 0 (Fatty Acid Transport Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Slc27a4 protein, mouse)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - OF5P57N2ZX (Alanine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - 3T3 Cells
MH  - Alanine/genetics
MH  - Amino Acid Motifs
MH  - Amino Acid Substitution/genetics
MH  - Animals
MH  - Carrier Proteins/*genetics/metabolism/physiology
MH  - Cell Line
MH  - Fatty Acid Transport Proteins
MH  - Fatty Acids/*metabolism
MH  - Glycine/genetics
MH  - Membrane Proteins/*genetics/metabolism/physiology
MH  - *Membrane Transport Proteins
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - *Point Mutation
MH  - Serine/genetics
MH  - Threonine/genetics
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - 10.1016/s0952-3278(99)80001-5 [doi]
PST - ppublish
SO  - Prostaglandins Leukot Essent Fatty Acids. 1999 May-Jun;60(5-6):285-9. doi:
      10.1016/s0952-3278(99)80001-5.