PMID- 10470852
OWN - NLM
STAT- MEDLINE
DCOM- 19991008
LR  - 20190513
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 6
IP  - 3
DP  - 1999 Jun 30
TI  - Structure of rat gamma-tubulin and its binding to HP33.
PG  - 207-9
AB  - Gamma-tubulin is localized at the microtubule organizing center and is thought to
      participate in the organizing of the microtubule network. In this study, we
      isolated a cDNA of rat gamma-tubulin. The rat gamma-tubulin cDNA encoded 451
      amino acids, the same number as that of its counterpart in other vertebrates, and
      its structure was found to be highly conserved in vertebrates. In a previous
      work, we identified HP33 (hepatocarcinogenesis- and hepatocellular
      proliferation-related 33-kDa protein) that was localized at the centrosome of
      hepatic cells and that exhibited MAP-like activity. In vitro GST pull-down assay 
      using highly purified recombinant HP33 and bacterially expressed gamma-tubulin
      demonstrated that HP33 bound to gamma-tubulin directly. These results suggest
      that HP33 is localized at the centrosome via association with both the
      microtubule and its minus end-specific component, gamma-tubulin.
FAU - Nakadai, T
AU  - Nakadai T
AD  - Department of Biology, Faculty of Science, Chiba University, Japan.
FAU - Okada, N
AU  - Okada N
FAU - Makino, Y
AU  - Makino Y
FAU - Tamura, T
AU  - Tamura T
LA  - eng
SI  - GENBANK/AB015946
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes
      and genomes
JID - 9423827
RN  - 0 (DNA, Complementary)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Tubulin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - DNA, Complementary/genetics
MH  - Gene Expression
MH  - Liver Neoplasms, Experimental/metabolism
MH  - Microtubule-Associated Proteins/*genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Rats
MH  - *Sequence Alignment
MH  - Tubulin/*genetics/*metabolism
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - 10.1093/dnares/6.3.207 [doi]
PST - ppublish
SO  - DNA Res. 1999 Jun 30;6(3):207-9. doi: 10.1093/dnares/6.3.207.