PMID- 10470077
OWN - NLM
STAT- MEDLINE
DCOM- 19990924
LR  - 20101118
IS  - 1078-8956 (Print)
IS  - 1078-8956 (Linking)
VI  - 5
IP  - 9
DP  - 1999 Sep
TI  - Targeted disruption of cd39/ATP diphosphohydrolase results in disordered
      hemostasis and thromboregulation.
PG  - 1010-7
AB  - CD39, or vascular adenosine triphosphate diphosphohydrolase, has been considered 
      an important inhibitor of platelet activation. Unexpectedly, cd39-deficient mice 
      had prolonged bleeding times with minimally perturbed coagulation parameters.
      Platelet interactions with injured mesenteric vasculature were considerably
      reduced in vivo and purified mutant platelets failed to aggregate to standard
      agonists in vitro. This platelet hypofunction was reversible and associated with 
      purinergic type P2Y1 receptor desensitization. In keeping with deficient vascular
      protective mechanisms, fibrin deposition was found at multiple organ sites in
      cd39-deficient mice and in transplanted cardiac grafts. Our data indicate a dual 
      role for adenosine triphosphate diphosphohydrolase in modulating hemostasis and
      thrombotic reactions.
FAU - Enjyoji, K
AU  - Enjyoji K
AD  - Department of Biology, Massachusetts Institute of Technology, Cambridge,
      Massachusetts 02139, USA.
FAU - Sevigny, J
AU  - Sevigny J
FAU - Lin, Y
AU  - Lin Y
FAU - Frenette, P S
AU  - Frenette PS
FAU - Christie, P D
AU  - Christie PD
FAU - Esch, J S 2nd
AU  - Esch JS 2nd
FAU - Imai, M
AU  - Imai M
FAU - Edelberg, J M
AU  - Edelberg JM
FAU - Rayburn, H
AU  - Rayburn H
FAU - Lech, M
AU  - Lech M
FAU - Beeler, D L
AU  - Beeler DL
FAU - Csizmadia, E
AU  - Csizmadia E
FAU - Wagner, D D
AU  - Wagner DD
FAU - Robson, S C
AU  - Robson SC
FAU - Rosenberg, R D
AU  - Rosenberg RD
LA  - eng
GR  - HL41002/HL/NHLBI NIH HHS/United States
GR  - HL57307/HL/NHLBI NIH HHS/United States
GR  - P01-41484/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - 0 (Antigens, CD)
RN  - 0 (P2ry1 protein, mouse)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2Y1)
RN  - 9001-31-4 (Fibrin)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.5 (Apyrase)
RN  - EC 3.6.1.5 (CD39 antigen)
SB  - IM
CIN - Nat Med. 1999 Sep;5(9):987-8. PMID: 10470068
MH  - *Adenosine Triphosphatases
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Apyrase/deficiency/genetics/*metabolism
MH  - Arterioles/pathology
MH  - Bleeding Time
MH  - *Blood Coagulation
MH  - Blood Platelets/cytology/pathology/*physiology
MH  - Cells, Cultured
MH  - Endothelium, Vascular/cytology/enzymology/metabolism
MH  - Female
MH  - Fibrin/metabolism
MH  - *Gene Deletion
MH  - Graft Rejection/immunology/pathology
MH  - Heart Transplantation/immunology/pathology
MH  - *Hemostasis
MH  - Male
MH  - Mesentery/blood supply
MH  - Mice
MH  - Mice, Knockout
MH  - Platelet Aggregation
MH  - Rats
MH  - Receptors, Purinergic P2/physiology
MH  - Receptors, Purinergic P2Y1
MH  - Thromboplastin/metabolism
MH  - Thrombosis/pathology
EDAT- 1999/09/02 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/09/02 09:00
PHST- 1999/09/02 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/09/02 09:00 [entrez]
AID - 10.1038/12447 [doi]
PST - ppublish
SO  - Nat Med. 1999 Sep;5(9):1010-7. doi: 10.1038/12447.