PMID- 10469656
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20161124
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 17
DP  - 1999 Sep 1
TI  - The nucleosomal response associated with immediate-early gene induction is
      mediated via alternative MAP kinase cascades: MSK1 as a potential histone
      H3/HMG-14 kinase.
PG  - 4779-93
AB  - The nucleosomal response refers to the rapid phosphorylation of histone H3 on
      serine 10 and HMG-14 on serine 6 that occurs concomitantly with immediate-early
      (IE) gene induction in response to a wide variety of stimuli. Using antibodies
      against the phosphorylated residues, we show that H3 and HMG-14 phosphorylation
      is mediated via different MAP kinase (MAPK) cascades, depending on the stimulus. 
      The nucleosomal response elicited by TPA is ERK-dependent, whereas that elicited 
      by anisomycin is p38 MAPK-dependent. In intact cells, the nucleosomal response
      can be selectively inhibited using the protein kinase inhibitor H89. MAPK
      activation and phosphorylation of transcription factors are largely unaffected by
      H89, whereas induction of IE genes is inhibited and its characteristics markedly 
      altered. MSK1 is considered the most likely kinase to mediate this response
      because (i) it is activated by both ERK and p38 MAPKs; (ii) it is an extremely
      efficient kinase for HMG-14 and H3, utilizing the physiologically relevant sites;
      and (iii) its activity towards H3/HMG-14 is uniquely sensitive to H89 inhibition.
      Thus, the nucleosomal response is an invariable consequence of ERK and p38 but
      not JNK/SAPK activation, and MSK1 potentially provides a link to complete the
      circuit between cell surface and nucleosome.
FAU - Thomson, S
AU  - Thomson S
AD  - Nuclear Signalling Laboratory, The Randall Institute, King's College, London,
      WC2B 5RL, UK.
FAU - Clayton, A L
AU  - Clayton AL
FAU - Hazzalin, C A
AU  - Hazzalin CA
FAU - Rose, S
AU  - Rose S
FAU - Barratt, M J
AU  - Barratt MJ
FAU - Mahadevan, L C
AU  - Mahadevan LC
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Histones)
RN  - 0 (Isoquinolines)
RN  - 0 (Nucleosomes)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Sulfonamides)
RN  - 6C74YM2NGI (Anisomycin)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 90-kDa)
RN  - EC 2.7.11.1 (mitogen and stress-activated protein kinase 1)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Anisomycin/pharmacology
MH  - Blotting, Western
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Fibroblasts/metabolism
MH  - *Gene Expression Regulation, Enzymologic
MH  - Genes, Immediate-Early/drug effects/*genetics
MH  - High Mobility Group Proteins/metabolism
MH  - Histones/*metabolism
MH  - Isoquinolines/pharmacology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Models, Biological
MH  - Nucleosomes/*metabolism
MH  - Phosphorylation
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - *Ribosomal Protein S6 Kinases, 90-kDa
MH  - Signal Transduction
MH  - *Sulfonamides
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Time Factors
MH  - Transcriptional Activation
PMC - PMC1171550
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - 10.1093/emboj/18.17.4779 [doi]
PST - ppublish
SO  - EMBO J. 1999 Sep 1;18(17):4779-93. doi: 10.1093/emboj/18.17.4779.