PMID- 10469594
OWN - NLM
STAT- MEDLINE
DCOM- 19991223
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 16
DP  - 1999 Aug 26
TI  - Blastocyst formation is blocked in mouse embryos lacking the splicing factor
      SRp20.
PG  - 899-902
AB  - SRp20 is a splicing factor belonging to the highly conserved family of SR
      proteins [1] [2] [3] [4], which have multiple roles in the regulation of
      constitutive and alternative splicing in vivo. It has been suggested that SR
      proteins are involved in bringing together the splice sites during spliceosome
      assembly [5]. SR proteins show partial redundancy, as each single SR protein can 
      restore splicing activity to a splicing-deficient cytoplasmic extract (termed
      S-100 extract). Nevertheless, several studies demonstrate that individual SR
      proteins have different effects on the selection of specific alternative splice
      sites, and they recognize distinct RNA sequences [6] [7] [8] [9] [10] [11] [12]. 
      Also, inactivation of two SR proteins, B52/SRp55 in Drosophila [13] or ASF/SF2 in
      the chicken cell line DT40 [14], is lethal, indicating the existence of
      nonredundant functions. Here, using Cre-loxP-mediated recombination in mice to
      inactivate the SRp20 gene, we found that it is essential for mouse development.
      Mutant preimplantation embryos failed to form blastocysts and died at the morula 
      stage. Immunofluorescent staining showed that SRp20 is present in oocytes and
      early stages of embryonic development. This is the first report of mice deficient
      for a member of the SR protein family. Our experiments confirm that, although
      similar in structure, the SR proteins are not functionally redundant.
FAU - Jumaa, H
AU  - Jumaa H
AD  - Max Planck Institute for Immunobiology, Stuebeweg 51, D-79108, Freiburg i. Br,
      Germany. jumaa@immunbio.mpg.de
FAU - Wei, G
AU  - Wei G
FAU - Nielsen, P J
AU  - Nielsen PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Srsf3 protein, mouse)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - *Blastocyst/chemistry
MH  - Electroporation
MH  - *Embryonic Development
MH  - Embryonic and Fetal Development/*physiology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Deletion
MH  - Genotype
MH  - Mice
MH  - Morula/chemistry
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - RNA-Binding Proteins/genetics/*physiology
MH  - Serine-Arginine Splicing Factors
MH  - Time Factors
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - S0960-9822(99)80394-7 [pii]
AID - 10.1016/s0960-9822(99)80394-7 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Aug 26;9(16):899-902. doi: 10.1016/s0960-9822(99)80394-7.