PMID- 10469298
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20161124
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 113
IP  - 2
DP  - 1999 Aug
TI  - Psoriasis upregulated phorbolin-1 shares structural but not functional similarity
      to the mRNA-editing protein apobec-1.
PG  - 162-9
AB  - Earlier studies of psoriatic and normal primary keratinocytes treated with
      phorbol 12-myristate-1-acetate identified two low-molecular-weight proteins,
      termed phorbolin-1 (20 kDa; pI 6.6) and phorbolin-2 (17.6 kDa; pI 6.5). As a
      first step towards elucidating the role of these proteins in psoriasis, we report
      here the molecular cloning and chromosomal mapping of phorbolin-1 and a related
      cDNA that codes for a protein exhibiting a similar amino acid sequence. The
      phorbolins were mapped to position 22q13 immediately centromeric to the c-sis
      proto-oncogene. Transient expression of the phorbolin-1 cDNA in COS cells and by 
      in vitro transcription/translation, yielded polypeptides that comigrated with
      phorbolins-1 and -2. Comparative sequence analysis revealed 22% overall identity 
      and a similarity of 44% of the phorbolins to apobec-1, the catalytic subunit of
      the mammalian apolipoprotein B mRNA editing enzyme; however,
      recombinant-expressed phorbolin-1 exhibited no cytidine deaminase activity, using
      either a monomeric nucleoside or apolipoprotein B cRNA as substrate, and failed
      to bind an AU-rich RNA template. Whereas the precise function of the phorbolins
      remains to be elucidated, the current data suggest that it is unlikely to include
      a role in the post-transcriptional modification of RNA in a manner analogous to
      that described for apobec-1.
FAU - Madsen, P
AU  - Madsen P
AD  - Department of Medical Biochemistry, University of Aarhus, Denmark.
      pm@biokemi.au.dk
FAU - Anant, S
AU  - Anant S
FAU - Rasmussen, H H
AU  - Rasmussen HH
FAU - Gromov, P
AU  - Gromov P
FAU - Vorum, H
AU  - Vorum H
FAU - Dumanski, J P
AU  - Dumanski JP
FAU - Tommerup, N
AU  - Tommerup N
FAU - Collins, J E
AU  - Collins JE
FAU - Wright, C L
AU  - Wright CL
FAU - Dunham, I
AU  - Dunham I
FAU - MacGinnitie, A J
AU  - MacGinnitie AJ
FAU - Davidson, N O
AU  - Davidson NO
FAU - Celis, J E
AU  - Celis JE
LA  - eng
SI  - GENBANK/U03891
GR  - DK-42030/DK/NIDDK NIH HHS/United States
GR  - HL-38180/HL/NHLBI NIH HHS/United States
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Proteins)
RN  - EC 3.5.4.- (AICDA (activation-induced cytidine deaminase))
RN  - EC 3.5.4.36 (APOBEC-1 Deaminase)
RN  - EC 3.5.4.36 (APOBEC1 protein, human)
RN  - EC 3.5.4.5 (APOBEC3A protein, human)
RN  - EC 3.5.4.5 (Cytidine Deaminase)
SB  - IM
MH  - APOBEC-1 Deaminase
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Cytidine Deaminase/chemistry/*physiology
MH  - Gene Library
MH  - Molecular Sequence Data
MH  - Proteins/*chemistry/genetics
MH  - Psoriasis/*physiopathology
MH  - Sequence Homology, Amino Acid
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - S0022-202X(15)40556-1 [pii]
AID - 10.1046/j.1523-1747.1999.00682.x [doi]
PST - ppublish
SO  - J Invest Dermatol. 1999 Aug;113(2):162-9. doi: 10.1046/j.1523-1747.1999.00682.x.