PMID- 10469296
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20061115
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 113
IP  - 2
DP  - 1999 Aug
TI  - Molecular cloning and tissue expression of the murine analog to human stratum
      corneum chymotryptic enzyme.
PG  - 152-5
AB  - Human stratum corneum chymotryptic enzyme (SCCE) may play a central part in
      epidermal homeostasis. Its proposed function is to catalyze the degradation of
      intercellular structures, including desmosomes, in the stratum corneum as part of
      the desquamation process. In order to facilitate physiologic and pathophysiologic
      studies on SCCE we have looked for the corresponding murine enzyme. A cDNA
      obtained by reverse transcription-polymerase chain reaction with total RNA
      prepared from mouse tails as starting material was cloned, and the expression of 
      the corresponding mRNA studied. The murine cDNA showed 77% homology to human SCCE
      cDNA. It had an open-reading frame encoding a protein comprising 249 amino acids 
      with 82% amino acid sequence homology to human SCCE including the conserved
      sequences of the catalytic traid of mammalian serine proteases. The murine
      protein was deduced to have a 21 amino acid signal peptide and a four amino acid 
      propeptide ending with a tryptic cleavage site, followed by a sequence motif
      identical to the N-terminal amino acid sequence of native active human SCCE. As
      in human SCCE the P2 position of the propeptide was occupied by an acidic amino
      acid residue, and the position corresponding to the suggested bottom of the
      primary substrate specificity pouch occupied by an asparagine residue. Analyses
      of mouse tissues by reverse transcriptase-polymerase chain reaction showed high
      expression in the skin, low expression in lung, kidney, brain, heart, and spleen,
      and no expression in liver or skeletal muscle. In situ hybridization of mouse
      skin showed expression in high suprabasal keratinocytes and in the luminal parts 
      of hair follicles. Our results strongly suggest that we have cloned the murine
      analog of human SCCE cDNA.
FAU - Backman, A
AU  - Backman A
AD  - Astra Hassle AB, Umea, Sweden.
FAU - Stranden, P
AU  - Stranden P
FAU - Brattsand, M
AU  - Brattsand M
FAU - Hansson, L
AU  - Hansson L
FAU - Egelrud, T
AU  - Egelrud T
LA  - eng
SI  - GENBANK/AF124299
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (DNA, Complementary)
RN  - EC 3.4.21.1 (Chymotrypsin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chymotrypsin/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Desmosomes/enzymology
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Skin/*enzymology/metabolism
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - 10.1046/j.1523-1747.1999.00662.x [doi]
AID - S0022-202X(15)40554-8 [pii]
PST - ppublish
SO  - J Invest Dermatol. 1999 Aug;113(2):152-5. doi: 10.1046/j.1523-1747.1999.00662.x.