PMID- 10469154
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 263
IP  - 3
DP  - 1999 Aug
TI  - High prevalence of 2-mono- and 2,6-di-substituted manol-terminating sequences
      among O-glycans released from brain glycopeptides by reductive alkaline
      hydrolysis.
PG  - 879-88
AB  - Di- to heptasaccharides isolated from total nondialyzable brain glycopeptides
      after release by alkaline borohydride treatment have been subjected to mass
      spectrometric and nuclear magnetic resonance spectroscopic analyses supplemented 
      by TLC-MS analyses of derived neoglycolipids. A family of Manol-terminating
      oligosaccharides has been revealed which includes novel sequences with a 2,
      6-disubstituted Manol: In contrast to the Manol-terminating HNK-1
      antigen-positive chains described previously that occur as a minor population
      [Yuen, C.-T., Chai, W., Loveless, R.W., Lawson, A.M., Margolis, R.U. & Feizi, T. 
      (1997) J. Biol. Chem. 272, 8924-8931], the above oligosaccharides are abundant.
      The ratio of these compounds to the classical
      N-acetylgalactosaminitol-terminating oligosaccharides is about 1 : 3. Thus, there
      appears to be in higher eukaryotes a major alternative pathway related to the
      yeast-type protein O-mannosylation, the enzymatic basis and functional importance
      of which now require investigation.
FAU - Chai, W
AU  - Chai W
AD  - The Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park
      Hospital, Harrow, Middlesex, UK.
FAU - Yuen, C T
AU  - Yuen CT
FAU - Kogelberg, H
AU  - Kogelberg H
FAU - Carruthers, R A
AU  - Carruthers RA
FAU - Margolis, R U
AU  - Margolis RU
FAU - Feizi, T
AU  - Feizi T
FAU - Lawson, A M
AU  - Lawson AM
LA  - eng
GR  - NS-13876/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Glycopeptides)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Oligosaccharides)
RN  - 3OWL53L36A (Mannitol)
SB  - IM
MH  - Animals
MH  - *Brain Chemistry
MH  - Carbohydrate Conformation
MH  - Carbohydrate Sequence
MH  - Chromatography, Ion Exchange
MH  - Chromatography, Thin Layer
MH  - Glycopeptides/*chemistry/isolation & purification
MH  - Indicators and Reagents
MH  - Mannitol/*analysis
MH  - Mass Spectrometry
MH  - Methylation
MH  - Molecular Sequence Data
MH  - Oligosaccharides/*chemistry/isolation & purification
MH  - Rabbits
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - ejb572 [pii]
AID - 10.1046/j.1432-1327.1999.00572.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Aug;263(3):879-88. doi: 10.1046/j.1432-1327.1999.00572.x.