PMID- 10469124
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 263
IP  - 3
DP  - 1999 Aug
TI  - Evidence that phospholipase C-gamma2 interacts with SLP-76, Syk, Lyn, LAT and the
      Fc receptor gamma-chain after stimulation of the collagen receptor glycoprotein
      VI in human platelets.
PG  - 612-23
AB  - Platelet activation by collagen is mediated by the sequential tyrosine
      phosphorylation of the Fc receptor gamma-chain (FcR gamma-chain), which is part
      of the collagen receptor glycoprotein VI, the tyrosine kinase Syk and
      phospholipase C-gamma2 (PLC-gamma2). In this study tyrosine-phosphorylated
      proteins that associate with PLC-gamma2 after stimulation by a collagen-related
      peptide (CRP) were characterized using glutathione S-transferase fusion proteins 
      of PLC-gamma2 Src homology (SH) domains and by immunoprecipitation of endogenous 
      PLC-gamma2. The majority of the tyrosine-phosphorylated proteins that associate
      with PLC-gamma2 bind to its C-terminal SH2 domain. These were found to include
      PLC-gamma2, Syk, SH2-domain-containing leucocyte protein of 76 kDa (SLP-76), Lyn,
      linker for activation of T cells (LAT) and the FcR gamma-chain. Direct
      association was detected between PLC-gamma2 and SLP-76, and between PLC-gamma2
      and LAT upon CRP stimulation of platelets by far-Western blotting. FcR
      gamma-chain and Lyn were found to co-immunoprecipitate with PLC-gamma2 as well as
      with unidentified 110-kDa and 75-kDa phosphoproteins. The absence of an in vivo
      association between Syk and PLC-gamma2 in platelets is in contrast with that for 
      PLC-gamma1 and Syk in B cells. The in vivo function of PLC-gamma2 SH2 domains was
      examined through measurement of Ca2+ increases in mouse megakaryocytes that had
      been microinjected with recombinant proteins. This revealed that the C-terminal
      SH2 domain is involved in the regulation of PLC-gamma2. These data indicate that 
      the C-terminal SH2 domain of PLC-gamma2 is important for PLC-gamma2 regulation
      through possible interactions with SLP-76, Syk, Lyn, LAT and the FcR gamma-chain.
FAU - Gross, B S
AU  - Gross BS
AD  - Department of Pharmacology, University of Oxford, UK.
FAU - Melford, S K
AU  - Melford SK
FAU - Watson, S P
AU  - Watson SP
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (LAT protein, human)
RN  - 0 (Lat protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (Syk protein, mouse)
RN  - EC 2.7.10.2 (lyn protein-tyrosine kinase)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - EC 3.4.21.5 (Thrombin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Blood Platelets/*metabolism
MH  - Calcium/metabolism
MH  - Carrier Proteins/*metabolism
MH  - Enzyme Precursors/*metabolism
MH  - Glutathione Transferase/metabolism
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Isoenzymes/*blood
MH  - Megakaryocytes/metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Phospholipase C gamma
MH  - Phosphoproteins/isolation & purification/*metabolism
MH  - Phosphorylation
MH  - Polymerase Chain Reaction
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, IgG/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Syk Kinase
MH  - Thrombin/metabolism
MH  - Type C Phospholipases/*blood
MH  - src Homology Domains
MH  - src-Family Kinases/*metabolism
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - ejb560 [pii]
AID - 10.1046/j.1432-1327.1999.00560.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Aug;263(3):612-23. doi: 10.1046/j.1432-1327.1999.00560.x.