PMID- 10468997
OWN - NLM
STAT- MEDLINE
DCOM- 20000509
LR  - 20190813
IS  - 0300-0664 (Print)
IS  - 0300-0664 (Linking)
VI  - 51
IP  - 2
DP  - 1999 Aug
TI  - Association of molecular variants of luteinizing hormone with menstrual
      disorders.
PG  - 243-6
AB  - OBJECTIVE: Luteinizing hormone (LH) promotes ovulation and luteinization of the
      ovarian follicle, and stimulates steroidogenesis in the ovaries. It is known to
      be present in different molecular forms, and secretion of abnormal LH has been
      implicated in menstrual disorders and infertility. The purpose of this study was 
      to determine any association of two recently described LH variants with menstrual
      disorders in Singapore Chinese women. One of these variants had Trp8 to Arg8 and 
      Ile15 to Thr15 replacements in the LH beta-subunit, while the second variant
      possessed Ser102 substitution for Gly102. PATIENTS: One hundred and seventy six
      patients with menstrual disorders and two hundred normal ovulatory women were
      recruited and screened for the presence of these two LH variants. METHODS: The
      polymerase chain reaction (PCR) products of patients were analysed by restriction
      fragment length polymorphism (RFLP) and the results were compared with those of
      normal ovulatory women and confirmed by DNA sequencing. RESULTS: Twenty one
      (11.9%) patients with menstrual disorders and twenty (10%) normal ovulatory women
      were found to carry the first variant, but its occurrence did not show any
      significant statistical difference between the patient and control groups (P =
      0.679). However, the second variant was only detected in seven (4%) patients with
      menstrual disorders, and none of the normal ovulatory subjects (P = 0.005).
      CONCLUSIONS: the study showed that the first variant was not associated with
      menstrual disorders, whereas the second variant might be implicated in menstrual 
      disorders in some Singapore Chinese women.
FAU - Ramanujam, L N
AU  - Ramanujam LN
AD  - Department of Obstetrics and Gynaecology, National University of Singapore,
      National University Hospital, Singapore.
FAU - Liao, W X
AU  - Liao WX
FAU - Roy, A C
AU  - Roy AC
FAU - Loganath, A
AU  - Loganath A
FAU - Goh, H H
AU  - Goh HH
FAU - Ng, S C
AU  - Ng SC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Endocrinol (Oxf)
JT  - Clinical endocrinology
JID - 0346653
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Adenoma/complications
MH  - Adolescent
MH  - Adult
MH  - Amenorrhea/etiology/genetics
MH  - Case-Control Studies
MH  - Chi-Square Distribution
MH  - Dysmenorrhea/genetics
MH  - Female
MH  - Humans
MH  - Luteinizing Hormone/*genetics
MH  - Menorrhagia/genetics
MH  - Menstruation Disturbances/*genetics
MH  - Oligomenorrhea/genetics
MH  - Pituitary Neoplasms/complications
MH  - *Point Mutation
MH  - Polycystic Ovary Syndrome/genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Primary Ovarian Insufficiency/genetics
MH  - Sequence Analysis, DNA
EDAT- 1999/09/01 09:00
MHDA- 2000/05/16 09:00
CRDT- 1999/09/01 09:00
PHST- 1999/09/01 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 1999/09/01 09:00 [entrez]
AID - cen791 [pii]
AID - 10.1046/j.1365-2265.1999.00791.x [doi]
PST - ppublish
SO  - Clin Endocrinol (Oxf). 1999 Aug;51(2):243-6. doi:
      10.1046/j.1365-2265.1999.00791.x.