PMID- 10468607
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 18
DP  - 1999 Aug 31
TI  - Recycling MHC class I molecules and endosomal peptide loading.
PG  - 10326-31
AB  - MHC class I molecules usually present peptides derived from endogenous antigens
      that are bound in the endoplasmic reticulum. Loading of exogenous antigens on
      class I molecules, e.g., in cross-priming, sometimes occurs, but the
      intracellular location where interaction between the antigenic fragment and class
      I takes place is unclear. Here we show that measles virus F protein can be
      presented by class I in transporters associated with antigen
      processing-independent, NH(4)Cl-sensitive manner, suggesting that class I
      molecules are able to interact and bind antigen in acidic compartments, like
      class II molecules. Studies on intracellular transport of green fluorescent
      protein-tagged class I molecules in living cells confirmed that a small fraction 
      of class I molecules indeed enters classical MHC class II compartments (MIICs)
      and is transported in MIICs back to the plasma membrane. Fractionation studies
      show that class I complexes in MIICs contain peptides. The pH in MIIC (around
      5.0) is such that efficient peptide exchange can occur. We thus present evidence 
      for a pathway for class I loading that is shared with class II molecules.
FAU - Gromme, M
AU  - Gromme M
AD  - Department of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121,
      1066 CX Amsterdam, The Netherlands.
FAU - Uytdehaag, F G
AU  - Uytdehaag FG
FAU - Janssen, H
AU  - Janssen H
FAU - Calafat, J
AU  - Calafat J
FAU - van Binnendijk, R S
AU  - van Binnendijk RS
FAU - Kenter, M J
AU  - Kenter MJ
FAU - Tulp, A
AU  - Tulp A
FAU - Verwoerd, D
AU  - Verwoerd D
FAU - Neefjes, J
AU  - Neefjes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (HLA-D Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - B-Lymphocytes/*immunology
MH  - Cell Line, Transformed
MH  - Cell Membrane/physiology
MH  - Endoplasmic Reticulum/*physiology
MH  - Endosomes/*physiology
MH  - Green Fluorescent Proteins
MH  - HLA-D Antigens/physiology
MH  - Herpesvirus 4, Human/genetics
MH  - Histocompatibility Antigens Class I/*physiology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Luminescent Proteins/metabolism
MH  - Measles virus/immunology
MH  - Recombinant Fusion Proteins/metabolism
MH  - Viral Fusion Proteins/metabolism
PMC - PMC17887
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - 10.1073/pnas.96.18.10326 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10326-31. doi:
      10.1073/pnas.96.18.10326.