PMID- 10467592
OWN - NLM
STAT- MEDLINE
DCOM- 19990929
LR  - 20081121
IS  - 1072-0502 (Print)
IS  - 1072-0502 (Linking)
VI  - 1
IP  - 2
DP  - 1994 Jul-Aug
TI  - Egr3/Pilot, a zinc finger transcription factor, is rapidly regulated by activity 
      in brain neurons and colocalizes with Egr1/zif268.
PG  - 140-52
AB  - Programs of gene activation may underlie long-term adaptive cellular responses to
      extracellular ligands. We have used a differential cDNA cloning strategy to
      identify genes that are strongly induced by excitatory stimuli in the adult rat
      hippocampus. Here, we report the rat cDNA sequence of a zinc-finger transcription
      factor, Egr3/Pilot, and characterize its regulated mRNA expression in brain. Egr3
      mRNA is rapidly and transiently induced in neurons of the hippocampus and cortex 
      by electroconvulsive seizure. mRNA levels peak 2 hr after the seizure and remain 
      elevated for as long as 8 hr. Egr3 mRNA is also rapidly induced in granule cells 
      of the dentate gyrus by synaptic NMDA receptor activation elicited by patterned
      stimulation of the perforant pathway and by drugs that alter dopamine
      neurotransmission in the striatum. Basal levels of Egr3 mRNA in the cortex appear
      to be driven by natural synaptic activity because monocular deprivation rapidly
      decreases Egr3 mRNA in the deafferented visual cortex. Aspects of the protein
      structure, sequence-specific DNA binding, transcriptional activity, and
      regulation of Egr3 are highly similar to another zinc-finger transcription
      factor, Egr1/zif268. Moreover, we demonstrate colocalization of Egr3 and zif268
      mRNAs in neurons of normal and stimulated cortex. Our studies suggest that
      interactions between these coregulated transcription factors may be important in 
      defining long-term, neuroplastic responses.
FAU - Yamagata, K
AU  - Yamagata K
AD  - Department of Neuroscience, Johns Hopkins University, School of Medicine,
      Baltimore, Maryland 21205, USA.
FAU - Kaufmann, W E
AU  - Kaufmann WE
FAU - Lanahan, A
AU  - Lanahan A
FAU - Papapavlou, M
AU  - Papapavlou M
FAU - Barnes, C A
AU  - Barnes CA
FAU - Andreasson, K I
AU  - Andreasson KI
FAU - Worley, P F
AU  - Worley PF
LA  - eng
GR  - AG09219/AG/NIA NIH HHS/United States
GR  - EY08900/EY/NEI NIH HHS/United States
GR  - EY09374/EY/NEI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Learn Mem
JT  - Learning & memory (Cold Spring Harbor, N.Y.)
JID - 9435678
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Egr1 protein, rat)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/cytology/physiology
MH  - Brain Chemistry/genetics/*physiology
MH  - DNA-Binding Proteins/*metabolism
MH  - Early Growth Response Protein 1
MH  - Gene Expression Regulation/*physiology
MH  - *Immediate-Early Proteins
MH  - In Situ Hybridization
MH  - Molecular Sequence Data
MH  - Neurons/*physiology
MH  - Rats
MH  - Retroviridae Proteins, Oncogenic/*metabolism
MH  - Transcription Factors/*genetics/metabolism
MH  - Transcriptional Activation
MH  - Zinc Fingers/*genetics
EDAT- 1994/07/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
PST - ppublish
SO  - Learn Mem. 1994 Jul-Aug;1(2):140-52.