PMID- 10467349
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20120613
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 6
IP  - 8
DP  - 1999 Aug
TI  - Overexpression of atypical PKC in PC12 cells enhances NGF-responsiveness and
      survival through an NF-kappaB dependent pathway.
PG  - 753-64
AB  - Removal of atypical PKC blocks NGF-induced differentiation of PC12 cells.1 We now
      examine the consequences that overexpression of atypical PKCs had upon NGF
      responses. PC12 cells were stably transfected with either PKC-iota or PKC-zeta.
      Overexpression of atypical PKCs markedly enhanced NGF- induced neurite outgrowth 
      as well as enhanced NGF-stimulated JNK kinase. Cotransfection of HA-JNK1 along
      with increasing concentrations of PKC-iota, resulted in dose-dependent
      phosphorylation of GST c-Jun (1 - 79). NGF treatment of PC12 cells resulted in
      activation of NF-kappaB. In comparison, overexpression of atypical PKC-iota was
      by itself sufficient to activate NF-kappaB and shift the kinetics of NGF-induced 
      kappaB activity. Furthermore, transfection of full-length antisense PKC-iota
      blocked basal and NGF-stimulated NF-kappaB. Differentiated and undifferentiated
      PC12 cells overexpressing atypical PKC-iota were protected from serum
      deprivation-induced cell death. Collectively, these findings demonstrate that
      atypical PKC-iota lies in a pathway that regulates NF-kappaB and contributes to
      both neurotrophin-mediated differentiation and survival signaling.
FAU - Wooten, M W
AU  - Wooten MW
AD  - Department of Biological Sciences, Auburn University, Alabama, USA.
      mwwooten@ag.auburn.edu
FAU - Seibenhener, M L
AU  - Seibenhener ML
FAU - Zhou, G
AU  - Zhou G
FAU - Vandenplas, M L
AU  - Vandenplas ML
FAU - Tan, T H
AU  - Tan TH
LA  - eng
GR  - 33661/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (Isoenzymes)
RN  - 0 (NF-kappa B)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.11.1 (protein kinase C zeta)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (protein kinase C lambda)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Gene Expression
MH  - Isoenzymes/*biosynthesis/genetics
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/*metabolism
MH  - Nerve Growth Factor/*metabolism/pharmacology
MH  - PC12 Cells
MH  - Protein Kinase C/*biosynthesis/genetics
MH  - Rats
MH  - *Signal Transduction
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - 10.1038/sj.cdd.4400548 [doi]
PST - ppublish
SO  - Cell Death Differ. 1999 Aug;6(8):753-64. doi: 10.1038/sj.cdd.4400548.