PMID- 10464311
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 36
DP  - 1999 Sep 3
TI  - cDNA cloning and chromosomal localization of human alpha(11) integrin. A
      collagen-binding, I domain-containing, beta(1)-associated integrin alpha-chain
      present in muscle tissues.
PG  - 25735-42
AB  - We previously identified a novel integrin alpha-chain in human fetal muscle cells
      (Gullberg, D., Velling, T., Sjoberg, G., and Sejersen, T. (1995) Dev. Dyn. 204,
      57-65). We have now isolated the full-length cDNA for this integrin subunit,
      alpha(11). The open reading frame of the cDNA encodes a precursor of 1188 amino
      acids. The predicted mature protein of 1166 amino acids contains seven conserved 
      FG-GAP repeats, an I domain with a metal ion-dependent adhesion site motif, a
      short transmembrane region, and a unique cytoplasmic domain of 24 amino acids
      containing the sequence GFFRS. alpha(11), like other I domain integrins, lacks a 
      dibasic cleavage site for generation of a heavy chain and a light chain, and it
      contains three potential divalent cation binding sites in repeats 5-7. The
      presence of 22 inserted amino acids in the extracellular stalk portion (amino
      acids 804-826) distinguishes the alpha(11) integrin sequence from other integrin 
      alpha-chains. Amino acid sequence comparisons reveal the highest identity of 42% 
      with the alpha(10) integrin chain. Immunoprecipitation with antibodies to
      alpha(11) integrin captures a 145-kDa protein distinctly larger than the 140-kDa 
      alpha(2) integrin chain when analyzed by SDS-polyacrylamide gel electrophoresis
      under nonreducing conditions. Fluorescence in situ hybridization maps the
      integrin alpha(11) gene to chromosome 15q23, in the vicinity of an identified
      locus for Bardet-Biedl syndrome. Based on Northern blotting, integrin alpha(11)
      mRNA levels are high in the adult human uterus and in the heart and intermediate 
      in skeletal muscle and some other tissues tested. During in vitro myogenic
      differentiation, alpha(11) mRNA and protein are up-regulated. Studies of ligand
      binding properties show that alpha(11)beta(1) binds collagen type I-Sepharose,
      and cultured muscle cells localize alpha(11)beta(1) into focal contacts on
      collagen type I. Future studies will reveal the importance of alpha(11)beta(1)
      for muscle development and integrity in adult muscle and other tissues.
FAU - Velling, T
AU  - Velling T
AD  - Department of Cell and Molecular Biology, BMC, Box 596, Uppsala University, S-751
      24 Uppsala, Sweden.
FAU - Kusche-Gullberg, M
AU  - Kusche-Gullberg M
FAU - Sejersen, T
AU  - Sejersen T
FAU - Gullberg, D
AU  - Gullberg D
LA  - eng
SI  - GENBANK/AF137378
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ITGA11 protein, human)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Integrins)
RN  - 9007-34-5 (Collagen)
SB  - IM
SB  - S
MH  - Adult
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 15
MH  - Cloning, Molecular
MH  - Collagen/metabolism
MH  - Female
MH  - *Genome, Human
MH  - Humans
MH  - *Integrin alpha Chains
MH  - Integrins/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/metabolism
MH  - Muscle, Smooth/metabolism
MH  - Myocardium/metabolism
EDAT- 1999/08/28 00:00
MHDA- 1999/08/28 00:01
CRDT- 1999/08/28 00:00
PHST- 1999/08/28 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1999/08/28 00:00 [entrez]
AID - 10.1074/jbc.274.36.25735 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 3;274(36):25735-42. doi: 10.1074/jbc.274.36.25735.