PMID- 10464238
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 36
DP  - 1999 Sep 3
TI  - Association of frabin with the actin cytoskeleton is essential for microspike
      formation through activation of Cdc42 small G protein.
PG  - 25197-200
AB  - We have recently isolated a novel actin filament-binding protein, named frabin.
      Frabin has one actin filament-binding domain (ABD), one Dbl homology domain
      (DHD), first pleckstrin homology domains (PHD) adjacent to DHD, one cysteine
      rich-domain (CRD), and second PHD from the N terminus to the C terminus in this
      order. Full-length frabin induces microspike formation and c-Jun N-terminal
      kinase (JNK) activation. We found here that the fragment of frabin containing DHD
      and first PHD stimulated guanine nucleotide exchange of Cdc42Hs small G protein, 
      but not that of RhoA or Rac1 small G protein. However, this fragment of frabin
      did not induce microspike formation, and ABD was additionally necessary for
      microspike formation. Frabin having ABD was associated with the actin
      cytoskeleton, whereas frabin lacking ABD was diffusely distributed in the
      cytoplasm. In contrast, ABD was not necessary for JNK activation but CRD and
      second PHD were additionally necessary for this activation. These results
      indicate that the association of frabin with the actin cytoskeleton is essential 
      for microspike formation but not for JNK activation and that different domains of
      frabin are involved in microspike formation and JNK activation through Cdc42
      activation.
FAU - Umikawa, M
AU  - Umikawa M
AD  - Department of Molecular Biology and Biochemistry, Osaka University Medical
      School, Suita 565-0871, Japan.
FAU - Obaishi, H
AU  - Obaishi H
FAU - Nakanishi, H
AU  - Nakanishi H
FAU - Satoh-Horikawa, K
AU  - Satoh-Horikawa K
FAU - Takahashi, K
AU  - Takahashi K
FAU - Hotta, I
AU  - Hotta I
FAU - Matsuura, Y
AU  - Matsuura Y
FAU - Takai, Y
AU  - Takai Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Actins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Peptide Fragments)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein, Saccharomyces cerevisiae)
SB  - IM
MH  - Actins/*metabolism
MH  - Animals
MH  - COS Cells
MH  - Cell Cycle Proteins/*metabolism
MH  - Cytoskeleton/metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Microfilament Proteins/*metabolism
MH  - Peptide Fragments/metabolism
MH  - Signal Transduction
MH  - cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
EDAT- 1999/08/28 00:00
MHDA- 1999/08/28 00:01
CRDT- 1999/08/28 00:00
PHST- 1999/08/28 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1999/08/28 00:00 [entrez]
AID - 10.1074/jbc.274.36.25197 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Sep 3;274(36):25197-200. doi: 10.1074/jbc.274.36.25197.