PMID- 10464175
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190513
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 11
IP  - 9
DP  - 1999 Sep
TI  - Assessment of Cmv1 candidates by genetic mapping and in vivo antibody depletion
      of NK cell subsets.
PG  - 1541-51
AB  - The mouse chromosome 6 locus Cmv1 controls resistance to infection with murine
      cytomegalovirus (MCMV). We have previously shown that Cmv1 is tightly linked to
      members of the NK gene complex (NKC) including the Ly49 gene family. To assess
      the candidacy of individual NKC members for the resistance locus, first we
      followed the co-segregation of Cd94, Nkg2d, and the well-characterized Ly49a,
      Ly49c and Ly49g genes with respect to Cmv1 in pre-existing panels of
      intraspecific backcross mice. Gene order and intergene distances (in cM) were:
      centromere-Cd94/Nkg2d-(0.05)-Ly49a/Ly49c/Ly49 g/Cmv1-(0.
      3)-Prp/Kap/D6Mit13/111/219. This result excludes Cd94 and Nkg2d as candidates
      whereas it localizes the Ly49 genes within the minimal genetic interval for Cmv1.
      Second, we monitored the cell surface expression of individual Ly49 receptors in 
      MCMV-infected mice over 2 weeks. The proportion of Ly49C(+) and Ly49C/I(+) cells 
      decreased, the proportion of Ly49A(+) and Ly49G2(+) remained constant, and the
      cell surface density of Ly49G2 increased during infection, suggesting that NK
      cell subsets might have different roles in the regulation of MCMV infection.
      Third, we performed in vivo antibody depletion of specific NK cell subsets.
      Depletion with single antibodies did not affect the resistant phenotype
      suggesting that Ly49A(+), Ly49C(+), Ly49G2(+) and Ly49C/I(+) populations are not 
      substantial players in MCMV resistance, and arguing for exclusion of the
      respective genes as candidates for Cmv1. In contrast, mice depleted with combined
      antibodies showed an intermediate phenotype. Whether residual NK cells,
      post-depletion, belong to a particular subset expressing another Ly49 receptor,
      or a molecule encoded by a yet to be identified gene of the NKC, is discussed.
FAU - Depatie, C
AU  - Depatie C
AD  - Department of Biochemistry, McGill University, Montreal, H3G 1Y6 Quebec, Canada.
FAU - Chalifour, A
AU  - Chalifour A
FAU - Pare, C
AU  - Pare C
FAU - Lee, S H
AU  - Lee SH
FAU - Vidal, S M
AU  - Vidal SM
FAU - Lemieux, S
AU  - Lemieux S
LA  - eng
SI  - GENBANK/G54774
SI  - GENBANK/G54856
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 0 (Antigens, Ly)
RN  - 0 (Antigens, Surface)
RN  - 0 (Lectins, C-Type)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily B)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity
MH  - Antigens/immunology
MH  - Antigens, Ly/*genetics
MH  - Antigens, Surface
MH  - Cell Culture Techniques
MH  - *Chromosome Mapping
MH  - Herpesviridae Infections/*genetics
MH  - Killer Cells, Natural/*immunology
MH  - Lectins, C-Type
MH  - Lymphocyte Count
MH  - *Lymphocyte Depletion
MH  - Lymphocyte Subsets/*immunology
MH  - Mice
MH  - Mice, Inbred A
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - *Muromegalovirus
MH  - NK Cell Lectin-Like Receptor Subfamily B
MH  - Proteins/immunology
EDAT- 1999/08/28 00:00
MHDA- 1999/08/28 00:01
CRDT- 1999/08/28 00:00
PHST- 1999/08/28 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1999/08/28 00:00 [entrez]
AID - 10.1093/intimm/11.9.1541 [doi]
PST - ppublish
SO  - Int Immunol. 1999 Sep;11(9):1541-51. doi: 10.1093/intimm/11.9.1541.